Oxygen contents, sulfate concentrations, and changes of IPLs under different storage conditions of samples from ODP Leg 204 and IODP Expedition 311
Cobertura |
MEDIAN LATITUDE: 47.466999 * MEDIAN LONGITUDE: -126.358755 * SOUTH-BOUND LATITUDE: 44.568530 * WEST-BOUND LONGITUDE: -127.000845 * NORTH-BOUND LATITUDE: 48.789583 * EAST-BOUND LONGITUDE: -125.073930 * DATE/TIME START: 2002-07-21T00:00:00 * DATE/TIME END: 2002-08-29T00:00:00 |
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Data(s) |
13/09/2010
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Resumo |
We have studied the effects of slow infiltration of oxygen on microbial communities in refrigerated legacy samples from ocean drilling expeditions. Storage was in heat-sealed, laminated foil bags with a N2 headspace for geomicrobiological studies. Analysis of microbial lipids suggests that Bacteria were barely detectable in situ but increased remarkably during storage. Detailed molecular examination of a methane-rich sediment horizon showed that refrigeration triggered selective growth of ANME-2 archaea and a drastic change in the bacterial community. Subsequent enrichment targeting methanogens yielded exclusively methylotrophs, which were probably selected for by high sulfate levels caused by oxidation of reduced sulfur species. We provide recommendations for sample storage in future ocean drilling expeditions. |
Formato |
application/zip, 5 datasets |
Identificador |
https://doi.pangaea.de/10.1594/PANGAEA.745155 doi:10.1594/PANGAEA.745155 |
Idioma(s) |
en |
Publicador |
PANGAEA |
Relação |
Lin, Yu-Shih (2009): In vitro study of microbial carbon cycling in subseafloor sediments. PhD Thesis, Elektronische Dissertationen an der Staats- und Universitätsbibliothek Bremen, Germany, urn:nbn:de:gbv:46-diss000117097 |
Direitos |
CC-BY: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted |
Fonte |
Supplement to: Lin, Yu-Shih; Biddle, Jennifer F; Lipp, Julius S; Orcutt, B; Holler, Thomas; Teske, Andreas P; Hinrichs, Kai-Uwe (2010): Effect of storage conditions on archaeal and bacterial communities in subsurface marine sediments. Geomicrobiology Journal, 27, 261-272, doi:10.1080/01490450903410423 |
Palavras-Chave | #[SO4]2-; 204-1249; 204-1249F; 204-1250; 204-1250E; 204-1251; 204-1251E; 311-U1325B; 311-U1326D; 311-U1327C; 311-U1328B; 311-U1328C; 311-U1329C; A; analyzed after storage; AR; Archaeal intact polar lipids; Archaeal IPL; Archaeol; B; Bacterial intact polar lipids; Bacterial IPL; Cascadia Margin Gas Hydrates; Center for Marine Environmental Sciences; coefficient of variation based on results of duplicate extractions; COMPCORE; Composite Core; Depth; DEPTH, sediment/rock; DRILL; Drilling/drill rig; Event; Exp311; frozen; Hydroxyarchaeols; Intact polar lipid; Integrated Ocean Drilling Program / International Ocean Discovery Program; IODP; Ion chromatography (Riedel et al. 2006); IPL; Joides Resolution; Label; Leg204; MARUM; Material; mcrA; Mean; MeOH; Methyl coenzyme reductase A; North Pacific Ocean; O; O2 content; Ocean Drilling Program; ODP; ODP sample designation; OH-AR; Oxygen; Photometry (Tabatabai, 1974); Prokaryotes; refrigerated; Sample code/label; see reference(s); shipboard data; Standard deviation; Std dev; Sulphate; Thermal conductive detector (TCD); Treatm; Treatment |
Tipo |
Dataset |