The Apis mellifera filamentous virus genome


Autoria(s): Gauthier, L; Cornman, S; Hartmann, U; Cousserans, F; Evans, J; de Miranda, J; Neumann, Peter
Data(s)

2015

Resumo

A complete reference genome of the Apis mellifera Filamentous virus (AmFV) was determined using Illumina Hiseq sequencing. The AmFV genome is a double stranded DNA molecule of approximately 498,500 nucleotides with a GC content of 50.8%. It encompasses 247 non-overlapping open reading frames (ORFs), equally distributed on both strands, which cover 65% of the genome. While most of the ORFs lacked threshold sequence alignments to reference protein databases, twenty-eight were found to display significant homologies with proteins present in other large double stranded DNA viruses. Remarkably, 13 ORFs had strong similarity with typical baculovirus domains such as PIFs (per os infectivity factor genes: pif-1, pif-2, pif-3 and p74) and BRO (Baculovirus Repeated Open Reading Frame). The putative AmFV DNA polymerase is of type B, but is only distantly related to those of the baculoviruses. The ORFs encoding proteins involved in nucleotide metabolism had the highest percent identity to viral proteins in GenBank. Other notable features include the presence of several collagen-like, chitin-binding, kinesin and pacifastin domains. Due to the large size of the AmFV genome and the inconsistent affiliation with other large double stranded DNA virus families infecting invertebrates, AmFV may belong to a new virus family.

Formato

application/pdf

Identificador

http://boris.unibe.ch/82558/1/viruses-07-02798.pdf

Gauthier, L; Cornman, S; Hartmann, U; Cousserans, F; Evans, J; de Miranda, J; Neumann, Peter (2015). The Apis mellifera filamentous virus genome. Viruses, 7(7), pp. 3798-3815. Molecular Diversity Preservation International MDPI 10.3390/v7072798 <http://dx.doi.org/10.3390/v7072798>

doi:10.7892/boris.82558

info:doi:10.3390/v7072798

info:pmid:26184284

urn:issn:1999-4915

Idioma(s)

eng

Publicador

Molecular Diversity Preservation International MDPI

Relação

http://boris.unibe.ch/82558/

Direitos

info:eu-repo/semantics/openAccess

Fonte

Gauthier, L; Cornman, S; Hartmann, U; Cousserans, F; Evans, J; de Miranda, J; Neumann, Peter (2015). The Apis mellifera filamentous virus genome. Viruses, 7(7), pp. 3798-3815. Molecular Diversity Preservation International MDPI 10.3390/v7072798 <http://dx.doi.org/10.3390/v7072798>

Palavras-Chave #590 Animals (Zoology) #630 Agriculture
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

PeerReviewed