A fast protocol for purification of translating mRNAs
| Data(s) |
25/03/2016
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|---|---|
| Resumo |
We present a protocol that enables easy extraction of translating mRNAs by purification of tagged ribosomes and associated mRNAs. By studying three different examples of stress influenced genes, we demonstrate the effectiveness and compare our rapid method to an older protocol (Halbeisen et al., 2009). |
| Formato |
application/pdf |
| Identificador |
http://boris.unibe.ch/80980/1/A%20fast%20protocol%20for%20purification.pdf Ross, Daniela; Altmann, Michael (2016). A fast protocol for purification of translating mRNAs. Methods, 107, pp. 57-62. Elsevier 10.1016/j.ymeth.2016.03.021 <http://dx.doi.org/10.1016/j.ymeth.2016.03.021> doi:10.7892/boris.80980 info:doi:10.1016/j.ymeth.2016.03.021 info:pmid:27020892 urn:issn:1046-2023 |
| Idioma(s) |
eng |
| Publicador |
Elsevier |
| Relação |
http://boris.unibe.ch/80980/ |
| Direitos |
info:eu-repo/semantics/restrictedAccess |
| Fonte |
Ross, Daniela; Altmann, Michael (2016). A fast protocol for purification of translating mRNAs. Methods, 107, pp. 57-62. Elsevier 10.1016/j.ymeth.2016.03.021 <http://dx.doi.org/10.1016/j.ymeth.2016.03.021> |
| Palavras-Chave | #570 Life sciences; biology #610 Medicine & health |
| Tipo |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion PeerReviewed |
