A synthetic histone pre-mRNA-U7 small nuclear RNA chimera undergoing cis cleavage in the cytoplasm of Xenopus oocytes.


Autoria(s): Stefanovic, B; Wittop Koning, T H; Schümperli, Daniel
Data(s)

25/08/1995

Resumo

The 3' processing of histone pre-mRNAs is a nuclear event in which the U7 small nuclear ribonucleoprotein (snRNP) participates as an essential trans-acting factor. We have constructed a chimeric histone-U7 RNA that when injected into the cytoplasm of Xenopus laevis oocytes assembles into a snRNP-like particle and becomes cleaved at the correct site(s). RNP assembly is a prerequisite for cleavage, but, since neither the RNA nor the RNP appreciably enter the nucleus, cleavage occurs mostly, if not exclusively, in the cytoplasm. Consistent with this, cleavage also occurs in enucleated oocytes or in oocytes which have been depleted of U7 snRNPs. Thus all necessary components for cleavage must be present in the oocyte cytoplasm. The novel cleavage occurs in cis, involving only a single molecule of chimeric RNA with its associated proteins. This reaction is equally dependent upon base pairing interactions between histone spacer sequences and the 5'-end of the U7 moiety as the natural in trans reaction. These results imply that U7 is the only snRNP required for histone RNA processing. Moreover, the chimeric RNA is expected to be useful for further studies of the cleavage and assembly mechanisms of U7 snRNP.

Formato

application/pdf

Identificador

http://boris.unibe.ch/77087/1/nar00016-0080.pdf

Stefanovic, B; Wittop Koning, T H; Schümperli, Daniel (1995). A synthetic histone pre-mRNA-U7 small nuclear RNA chimera undergoing cis cleavage in the cytoplasm of Xenopus oocytes. Nucleic acids research, 23(16), pp. 3152-3160. Information Retrieval Ltd.

doi:10.7892/boris.77087

info:pmid:7667091

urn:issn:0305-1048

Idioma(s)

eng

Publicador

Information Retrieval Ltd.

Relação

http://boris.unibe.ch/77087/

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Stefanovic, B; Wittop Koning, T H; Schümperli, Daniel (1995). A synthetic histone pre-mRNA-U7 small nuclear RNA chimera undergoing cis cleavage in the cytoplasm of Xenopus oocytes. Nucleic acids research, 23(16), pp. 3152-3160. Information Retrieval Ltd.

Palavras-Chave #570 Life sciences; biology
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

PeerReviewed