Angiogenesis-related ultrastructural changes to capillaries in human skeletal muscle in response to endurance exercise


Autoria(s): Baum, Oliver; Gübeli, Jennifer; Frese, Sebastian; Torchetti, Eleonora; Malik, Corinna; Odriozola Quesada, Adolfo; Graber, Franziska; Hoppeler, Hans-Heinrich; Tschanz, Stefan A.
Data(s)

15/11/2015

31/12/1969

Resumo

The ultrastructure of capillaries in skeletal muscle was morphometrically assessed in vastus lateralis muscle (VL) biopsies taken before and after exercise from 22 participants of two training studies. In study 1 (8 wk of ergometer training), light microscopy revealed capillary-fiber (C/F) ratio (+27%) and capillary density (+16%) to be higher (P ≤ 0.05) in postexercise biopsies than in preexercise biopsies from all 10 participants. In study 2 (6 mo of moderate running), C/F ratio and capillary density were increased (+23% and +20%; respectively, P ≤ 0.05) in VL biopsies from 6 angiogenesis responders (AR) after training, whereas 6 nonangiogenesis responders (NR) showed nonsignificant changes in these structural indicators (-4%/-4%, respectively). Forty capillary profiles per participant were evaluated by point and intersection counting on cross sections after transmission electron microscopy. In study 1, volume density (Vv) and mean arithmetic thickness (T) of endothelial cells (ECs; +19%/+17%, respectively) and pericytes (PCs; +20%/+21%, respectively) were higher (P ≤ 0.05), whereas Vv and T of the pericapillary basement membrane (BM) were -23%/-22% lower (P ≤ 0.05), respectively, in posttraining biopsies. In study 2, exercise-related differences between AR and NR-groups were found for Vv and T of PCs (AR, +26%/+22%, respectively, both P ≤ 0.05; NR, +1%/-3%, respectively, both P > 0.05) and BM (AR, -14%/-13%, respectively, both P ≤ 0.05; NR, -9%/-11%, respectively, P = 0.07/0.10). Vv and T of ECs were higher (AR, +16%/+18%, respectively; NR, +6%/+6%, respectively; all P ≤ 0.05) in both groups. The PC coverage was higher (+13%, P ≤ 0.05) in VL biopsies of individuals in the AR group but nonsignificantly altered (+3%, P > 0.05) in those of the NR group after training. Our study suggests that intensified PC mobilization and BM thinning are related to exercise-induced angiogenesis in human skeletal muscle, whereas training per se induces EC-thickening.

Formato

application/pdf

Identificador

http://boris.unibe.ch/75241/1/1118.full.pdf

Baum, Oliver; Gübeli, Jennifer; Frese, Sebastian; Torchetti, Eleonora; Malik, Corinna; Odriozola Quesada, Adolfo; Graber, Franziska; Hoppeler, Hans-Heinrich; Tschanz, Stefan A. (2015). Angiogenesis-related ultrastructural changes to capillaries in human skeletal muscle in response to endurance exercise. Journal of applied physiology, 119(10), pp. 1118-1126. American Physiological Society 10.1152/japplphysiol.00594.2015 <http://dx.doi.org/10.1152/japplphysiol.00594.2015>

doi:10.7892/boris.75241

info:doi:10.1152/japplphysiol.00594.2015

info:pmid:26384412

urn:issn:8750-7587

Idioma(s)

eng

Publicador

American Physiological Society

Relação

http://boris.unibe.ch/75241/

Direitos

info:eu-repo/semantics/openAccess

Fonte

Baum, Oliver; Gübeli, Jennifer; Frese, Sebastian; Torchetti, Eleonora; Malik, Corinna; Odriozola Quesada, Adolfo; Graber, Franziska; Hoppeler, Hans-Heinrich; Tschanz, Stefan A. (2015). Angiogenesis-related ultrastructural changes to capillaries in human skeletal muscle in response to endurance exercise. Journal of applied physiology, 119(10), pp. 1118-1126. American Physiological Society 10.1152/japplphysiol.00594.2015 <http://dx.doi.org/10.1152/japplphysiol.00594.2015>

Palavras-Chave #610 Medicine & health
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

PeerReviewed