Quantitative PCR for the diagnosis of cutaneous leishmaniasis from formalin-fixed and paraffin-embedded skin sections.
| Data(s) |
01/12/2015
31/12/1969
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| Resumo |
The present report describes a real-time PCR-based procedure to reliably determine the quantity of Leishmania amastigotes in relation to the amount of host tissue in histological skin sections from canine and equine cases of cutaneous leishmaniasis. The novel diagnostic Leishmania-PCR has a detection limit of <0.02 amastigotes per μg tissue, which corresponds well to the detection limit of immunohistochemistry and is far beyond that of conventional histology. Our results emphasise the importance of PCR to complement routine histology of cutaneous leishmaniasis cases, particularly in laboratories in which no immunohistochemical assay is available. |
| Formato |
application/pdf application/pdf |
| Identificador |
http://boris.unibe.ch/75146/8/1-s2.0-S0890850815300414-main.pdf Müller, Norbert; Hentrich, Brigitte; Frey, Caroline; Welle, Monika Maria (2015). Quantitative PCR for the diagnosis of cutaneous leishmaniasis from formalin-fixed and paraffin-embedded skin sections. Molecular and cellular probes, 29(6), pp. 507-510. Elsevier 10.1016/j.mcp.2015.09.008 <http://dx.doi.org/10.1016/j.mcp.2015.09.008> doi:10.7892/boris.75146 info:doi:10.1016/j.mcp.2015.09.008 info:pmid:26427730 urn:issn:0890-8508 |
| Idioma(s) |
eng |
| Publicador |
Elsevier |
| Relação |
http://boris.unibe.ch/75146/ |
| Direitos |
info:eu-repo/semantics/openAccess info:eu-repo/semantics/restrictedAccess |
| Fonte |
Müller, Norbert; Hentrich, Brigitte; Frey, Caroline; Welle, Monika Maria (2015). Quantitative PCR for the diagnosis of cutaneous leishmaniasis from formalin-fixed and paraffin-embedded skin sections. Molecular and cellular probes, 29(6), pp. 507-510. Elsevier 10.1016/j.mcp.2015.09.008 <http://dx.doi.org/10.1016/j.mcp.2015.09.008> |
| Palavras-Chave | #610 Medicine & health #630 Agriculture |
| Tipo |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion PeerReviewed |
