Time Course of Antibiotic and Antifungal Concentrations in Corneal Organ Culture.


Autoria(s): Seiler, Günter Theodor Michael; Tschopp, Markus; Zimmerli, Stefan; Tappeiner, Christoph; Wittwer, Valéry; Früh Epstein, Beatrice
Data(s)

06/11/2015

31/12/1969

Resumo

PURPOSE Contamination with bacteria and/or fungi is a serious complication in organ-cultured corneas. Hence, antibiotic and antifungal agents are added to the culture medium. The concentration of different antimicrobial and antifungal additives to the media over time has so far not been investigated in detail and is the aim of this study. METHODS Nine human fresh corneoscleral discs were stored in corneal culture medium consisting of 2% fetal bovine serum and minimal essential medium. In addition, the culture medium contained 1200 μg/mL penicillin G, 25 μg/mL amphotericin B, 120 μg/mL streptomycin, and 100 μg/mL voriconazole. The concentration of amphotericin B used was 10 times higher than in clinical routine to facilitate its detection. The cultures were kept at 37°C for 28 days. At days 0, 7, 14, 21, and 28, samples of the culture medium were harvested for analysis of antimicrobial concentrations by liquid chromatography and electrospray ionization tandem mass spectrometry. RESULTS During corneal storage, the concentration of all antibiotics and antifungal agents declined significantly. By day 28, penicillin G was reduced to 14% of the original concentration. Amphotericin B and streptomycin retained approximately 60% of the original concentration to the end of the experiment and voriconazole maintained stable concentrations after an initial decline to approximately 80% at 7 days. CONCLUSIONS Throughout the entire storage period, the concentrations of penicillin G, streptomycin, and voriconazole exceeded the minimum inhibitory concentrations of all common contaminants, obviating the need for a change of the medium for antimicrobial reasons. Based on the minimum inhibitory concentrations and our findings, the initial concentration of amphotericin B should be raised to 5 μg/mL.

Formato

application/pdf

Identificador

http://boris.unibe.ch/73300/1/00003226-201601000-00022.pdf

Seiler, Günter Theodor Michael; Tschopp, Markus; Zimmerli, Stefan; Tappeiner, Christoph; Wittwer, Valéry; Früh Epstein, Beatrice (2015). Time Course of Antibiotic and Antifungal Concentrations in Corneal Organ Culture. Cornea, 35(1), pp. 127-131. Lippincott Williams & Wilkins 10.1097/ICO.0000000000000671 <http://dx.doi.org/10.1097/ICO.0000000000000671>

doi:10.7892/boris.73300

info:doi:10.1097/ICO.0000000000000671

info:pmid:26555594

urn:issn:0277-3740

Idioma(s)

eng

Publicador

Lippincott Williams & Wilkins

Relação

http://boris.unibe.ch/73300/

Direitos

info:eu-repo/semantics/embargoedAccess

Fonte

Seiler, Günter Theodor Michael; Tschopp, Markus; Zimmerli, Stefan; Tappeiner, Christoph; Wittwer, Valéry; Früh Epstein, Beatrice (2015). Time Course of Antibiotic and Antifungal Concentrations in Corneal Organ Culture. Cornea, 35(1), pp. 127-131. Lippincott Williams & Wilkins 10.1097/ICO.0000000000000671 <http://dx.doi.org/10.1097/ICO.0000000000000671>

Palavras-Chave #610 Medicine & health
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

PeerReviewed