Cold Microwave-Enabled Protein Detection and Quantification.
| Contribuinte(s) |
Kurien, Bijt T. Scofield, Hal R. |
|---|---|
| Data(s) |
2015
|
| Resumo |
Protein screening/detection is an essential tool in many laboratories. Owing to the relatively large time investments that are required by standard protocols, the development of methods with higher throughput while maintaining an at least comparable signal-to-noise ratio is highly beneficial in many research areas. This chapter describes how cold microwave technology can be used to enhance the rate of molecular interactions and provides protocols for dot blots, Western blots, and ELISA procedures permitting a completion of all incubation steps (blocking and antibody steps) within 24-45 min. |
| Formato |
application/pdf |
| Identificador |
Grützner, Niels; Heilmann, Romy M; Smith, Aaron G; Johnson, Carol B; Vitha, Stanislav; Steiner, Jörg M; Holzenburg, Andreas K (2015). Cold Microwave-Enabled Protein Detection and Quantification. In: Kurien, Bijt T.; Scofield, Hal R. (eds.) Detection of Blotted Proteins. Methods and Protocols. Methods in Molecular Biology: Vol. 1314 (pp. 207-217). New York: Humana Press 10.1007/978-1-4939-2718-0_23 <http://dx.doi.org/10.1007/978-1-4939-2718-0_23> doi:10.7892/boris.72206 info:doi:10.1007/978-1-4939-2718-0_23 info:pmid:26139270 urn:issn:1064-3745 urn:isbn:978-1-4939-2717-3 |
| Idioma(s) |
eng |
| Publicador |
Humana Press |
| Relação |
http://boris.unibe.ch/72206/ |
| Direitos |
info:eu-repo/semantics/restrictedAccess |
| Fonte |
Grützner, Niels; Heilmann, Romy M; Smith, Aaron G; Johnson, Carol B; Vitha, Stanislav; Steiner, Jörg M; Holzenburg, Andreas K (2015). Cold Microwave-Enabled Protein Detection and Quantification. In: Kurien, Bijt T.; Scofield, Hal R. (eds.) Detection of Blotted Proteins. Methods and Protocols. Methods in Molecular Biology: Vol. 1314 (pp. 207-217). New York: Humana Press 10.1007/978-1-4939-2718-0_23 <http://dx.doi.org/10.1007/978-1-4939-2718-0_23> |
| Palavras-Chave | #630 Agriculture #570 Life sciences; biology |
| Tipo |
info:eu-repo/semantics/bookPart info:eu-repo/semantics/publishedVersion PeerReviewed |
