Molecular characterization and chromosomal assignment of the bovine glycinamide ribonucleotide formyltransferase (GART) gene on cattle chromosome 1q12.1-q12.2


Autoria(s): Wöhlke, Anne; Drögemüller, Cord; Kuiper, Heidi; Leeb, Tosso; Distl, Ottmar
Data(s)

28/03/2005

Resumo

The mammalian glycinamide ribonucleotide formyltransferase (GART) genes encode a trifunctional polypeptide involved in the de novo purine biosynthesis. We isolated a bacterial artificial chromosome (BAC) clone containing the bovine GART gene and determined the complete DNA sequence of the BAC clone. Cloning and characterization of the bovine GART gene revealed that the bovine gene consists of 23 exons spanning approximately 27 kb. RT-PCR amplification of bovine GART in different organs showed the expression of two GART transcripts in cattle similar to human and mouse. The GART transcripts encode two proteins of 1010 and 433 amino acids, respectively. Eleven single nucleotide polymorphisms (SNPs) were detected in a mutation scan of 24 unrelated animals of three different cattle breeds, including one SNP that affects the amino acid sequence of GART. The chromosomal localization of the gene was determined by fluorescence in situ hybridization. Comparative genome analysis between cattle, human and mouse indicates that the chromosomal location of the bovine GART gene is in agreement with a previously published mapping report.

Formato

application/pdf

Identificador

http://boris.unibe.ch/70803/1/1-s2.0-S037811190400784X-main.pdf

Wöhlke, Anne; Drögemüller, Cord; Kuiper, Heidi; Leeb, Tosso; Distl, Ottmar (2005). Molecular characterization and chromosomal assignment of the bovine glycinamide ribonucleotide formyltransferase (GART) gene on cattle chromosome 1q12.1-q12.2. Gene, 348, pp. 73-81. Elsevier 10.1016/j.gene.2004.12.038 <http://dx.doi.org/10.1016/j.gene.2004.12.038>

doi:10.7892/boris.70803

info:doi:10.1016/j.gene.2004.12.038

info:pmid:15777723

urn:issn:0378-1119

Idioma(s)

eng

Publicador

Elsevier

Relação

http://boris.unibe.ch/70803/

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Wöhlke, Anne; Drögemüller, Cord; Kuiper, Heidi; Leeb, Tosso; Distl, Ottmar (2005). Molecular characterization and chromosomal assignment of the bovine glycinamide ribonucleotide formyltransferase (GART) gene on cattle chromosome 1q12.1-q12.2. Gene, 348, pp. 73-81. Elsevier 10.1016/j.gene.2004.12.038 <http://dx.doi.org/10.1016/j.gene.2004.12.038>

Palavras-Chave #590 Animals (Zoology) #630 Agriculture #570 Life sciences; biology
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

PeerReviewed