The integrity of the G2421-C2395 base pair in the ribosomal E-site is crucial for protein synthesis.


Autoria(s): Koch, Miriam; Clementi, Nina; Rusca, Nicola; Vögele, Paul; Erlacher, Matthias; Polacek, Norbert
Data(s)

02/01/2015

Resumo

During the elongation cycle of protein biosynthesis, tRNAs traverse through the ribosome by consecutive binding to the 3 ribosomal binding sites (A-, P-, and E- sites). While the ribosomal A- and P-sites have been functionally well characterized in the past, the contribution of the E-site to protein biosynthesis is still poorly understood in molecular terms. Previous studies suggested an important functional interaction of the terminal residue A76 of E-tRNA with the nucleobase of the universally conserved 23S rRNA residue C2394. Using an atomic mutagenesis approach to introduce non-natural nucleoside analogs into the 23S rRNA, we could show that removal of the nucleobase or the ribose 2'-OH at C2394 had no effect on protein synthesis. On the other hand, our data disclose the importance of the highly conserved E-site base pair G2421-C2395 for effective translation. Ribosomes with a disrupted G2421-C2395 base pair are defective in tRNA binding to the E-site. This results in an impaired translation of genuine mRNAs, while homo-polymeric templates are not affected. Cumulatively our data emphasize the importance of E-site tRNA occupancy and in particular the intactness of the 23S rRNA base pair G2421-C2395 for productive protein biosynthesis.

Formato

application/pdf

Identificador

http://boris.unibe.ch/67761/1/Koch_RNA%20Biol_2015.pdf

Koch, Miriam; Clementi, Nina; Rusca, Nicola; Vögele, Paul; Erlacher, Matthias; Polacek, Norbert (2015). The integrity of the G2421-C2395 base pair in the ribosomal E-site is crucial for protein synthesis. RNA biology, 12(1), pp. 70-81. Taylor and Francis 10.1080/15476286.2015.1017218 <http://dx.doi.org/10.1080/15476286.2015.1017218>

doi:10.7892/boris.67761

info:doi:10.1080/15476286.2015.1017218

info:pmid:25826414

urn:issn:1555-8584

Idioma(s)

eng

Publicador

Taylor and Francis

Relação

http://boris.unibe.ch/67761/

Direitos

info:eu-repo/semantics/openAccess

Fonte

Koch, Miriam; Clementi, Nina; Rusca, Nicola; Vögele, Paul; Erlacher, Matthias; Polacek, Norbert (2015). The integrity of the G2421-C2395 base pair in the ribosomal E-site is crucial for protein synthesis. RNA biology, 12(1), pp. 70-81. Taylor and Francis 10.1080/15476286.2015.1017218 <http://dx.doi.org/10.1080/15476286.2015.1017218>

Palavras-Chave #570 Life sciences; biology #540 Chemistry
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

PeerReviewed