On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7
| Data(s) |
2014
|
|---|---|
| Resumo |
Strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-β-carotene to form a supposedly 9-cis-configured β-apo-10′-carotenal. CCD8 converts this intermediate through a combination of yet undetermined reactions into the strigolactone-like compound carlactone. Here, we investigated the substrate and stereo-specificity of the Arabidopsis and pea CCD7 and determined the stereo-configuration of the β-apo-10′-carotenal intermediate by using Nuclear Magnetic Resonance Spectroscopy. Our data unequivocally demonstrate the 9-cis-configuration of the intermediate. Both CCD7s cleave different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10′-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-β-carotene. |
| Formato |
application/pdf |
| Identificador |
http://boris.unibe.ch/67526/1/1-s2.0-S0014579314002609-main.pdf Bruno, Mark; Hofmann, Manuel; Vermathen, Martina; Alder, Adrian; Beyer, Peter; Al-Babili, Salim (2014). On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7. FEBS letters, 588(9), pp. 1802-1807. Elsevier 10.1016/j.febslet.2014.03.041 <http://dx.doi.org/10.1016/j.febslet.2014.03.041> doi:10.7892/boris.67526 info:doi:10.1016/j.febslet.2014.03.041 urn:issn:0014-5793 |
| Idioma(s) |
eng |
| Publicador |
Elsevier |
| Relação |
http://boris.unibe.ch/67526/ |
| Direitos |
info:eu-repo/semantics/restrictedAccess |
| Fonte |
Bruno, Mark; Hofmann, Manuel; Vermathen, Martina; Alder, Adrian; Beyer, Peter; Al-Babili, Salim (2014). On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7. FEBS letters, 588(9), pp. 1802-1807. Elsevier 10.1016/j.febslet.2014.03.041 <http://dx.doi.org/10.1016/j.febslet.2014.03.041> |
| Palavras-Chave | #570 Life sciences; biology #540 Chemistry |
| Tipo |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion PeerReviewed |
