The role of microclot formation in an acute subarachnoid hemorrhage model in the rabbit
Data(s) |
2014
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Resumo |
BACKGROUND Microvascular dysfunction and microthrombi formation are believed to contribute to development of early brain injury (EBI) after aneurysmal subarachnoid hemorrhage (SAH). OBJECTIVE This study aimed to determine (i) extent of microthrombus formation and neuronal apoptosis in the brain parenchyma using a blood shunt SAH model in rabbits; (ii) correlation of structural changes in microvessels with EBI characteristics. METHODS Acute SAH was induced using a rabbit shunt cisterna magna model. Extent of microthrombosis was detected 24 h post-SAH (n = 8) by fibrinogen immunostaining, compared to controls (n = 4). We assessed apoptosis by terminal deoxynucleotidyl transferase nick end labeling (TUNEL) in cortex and hippocampus. RESULTS Our results showed significantly more TUNEL-positive cells (SAH: 115 ± 13; controls: 58 ± 10; P = 0.016) and fibrinogen-positive microthromboemboli (SAH: 9 ± 2; controls: 2 ± 1; P = 0.03) in the hippocampus after aneurysmal SAH. CONCLUSIONS We found clear evidence of early microclot formation in a rabbit model of acute SAH. The extent of microthrombosis did not correlate with early apoptosis or CPP depletion after SAH; however, the total number of TUNEL positive cells in the cortex and the hippocampus significantly correlated with mean CPP reduction during the phase of maximum depletion after SAH induction. Both microthrombosis and neuronal apoptosis may contribute to EBI and subsequent DCI. |
Formato |
application/pdf |
Identificador |
Andereggen, Lukas; Neuschmelting, Volker; von Gunten, Michael; Widmer, Hans Rudolf; Fandino, Javier; Marbacher, Serge (2014). The role of microclot formation in an acute subarachnoid hemorrhage model in the rabbit. BioMed research international, 2014, p. 161702. Hindawi Publishing Corporation 10.1155/2014/161702 <http://dx.doi.org/10.1155/2014/161702> doi:10.7892/boris.65150 info:doi:10.1155/2014/161702 info:pmid:25110658 urn:issn:2314-6133 |
Idioma(s) |
eng |
Publicador |
Hindawi Publishing Corporation |
Relação |
http://boris.unibe.ch/65150/ |
Direitos |
info:eu-repo/semantics/openAccess |
Fonte |
Andereggen, Lukas; Neuschmelting, Volker; von Gunten, Michael; Widmer, Hans Rudolf; Fandino, Javier; Marbacher, Serge (2014). The role of microclot formation in an acute subarachnoid hemorrhage model in the rabbit. BioMed research international, 2014, p. 161702. Hindawi Publishing Corporation 10.1155/2014/161702 <http://dx.doi.org/10.1155/2014/161702> |
Palavras-Chave | #610 Medicine & health |
Tipo |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion PeerReviewed |