Optogenetics: opsins and optical interfaces in neuroscience.


Autoria(s): Adamantidis, Antoine Roger; Zhang, Feng; de Lecea, Luis; Deisseroth, Karl
Data(s)

01/08/2014

Resumo

Optogenetics is defined as the integration of optics and genetics to control well-defined events within specified cells of living tissue. In this introduction, we focus on the basic techniques necessary for employing microbial opsins as optogenetic tools in mammalian brains. We provide a guide for the fundamentals of optogenetic application-selecting an opsin, implementing expression of opsins based on the neuroscientific experimental requirements, and adapting the corresponding optical hardware for delivery of light into mammalian brains.

Formato

application/pdf

Identificador

http://boris.unibe.ch/63670/1/2014%20AA%20CSH%20Protocols.pdf

Adamantidis, Antoine Roger; Zhang, Feng; de Lecea, Luis; Deisseroth, Karl (2014). Optogenetics: opsins and optical interfaces in neuroscience. Cold Spring Harbor protocols, 2014(8), pp. 815-822. Cold Spring Harbor Laboratory 10.1101/pdb.top083329 <http://dx.doi.org/10.1101/pdb.top083329>

doi:10.7892/boris.63670

info:doi:10.1101/pdb.top083329

info:pmid:25086025

urn:issn:1940-3402

Idioma(s)

eng

Publicador

Cold Spring Harbor Laboratory

Relação

http://boris.unibe.ch/63670/

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Adamantidis, Antoine Roger; Zhang, Feng; de Lecea, Luis; Deisseroth, Karl (2014). Optogenetics: opsins and optical interfaces in neuroscience. Cold Spring Harbor protocols, 2014(8), pp. 815-822. Cold Spring Harbor Laboratory 10.1101/pdb.top083329 <http://dx.doi.org/10.1101/pdb.top083329>

Palavras-Chave #610 Medicine & health
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

PeerReviewed