Immunoblotting for the serodiagnosis of alveolar echinococcosis in alive and dead Eurasian beavers (Castor fiber)
| Data(s) |
15/09/2014
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| Resumo |
A novel species-specific anti-beaver-IgG-alkaline-phosphatase conjugate was synthesized for the development of a new serological test for echinococcosis in beavers. Two different ELISAs conventionally used for human Echinococcus multilocularis serology (Em18-ELISA and Em2-ELISA) yielded diagnostic sensitivities of 0% and 46%, respectively. In contrast, the subsequently developed immunoblotting assay gave an 85% diagnostic sensitivity (11 out of 13 beavers with alveolar echinococcosis were immunoblotting-positive, i.e. showed reactivity with a specific 21 Mr band), and maximal specificity. In conclusion, this immunoblotting assay should be the method of choice for use in serological studies on E. multilocularis in Eurasian beavers, and the test proved suitable to investigate both animals alive and post-mortem. |
| Formato |
application/pdf application/pdf |
| Identificador |
http://boris.unibe.ch/63067/1/1-s2.0-S0304401714003549-main.pdf http://boris.unibe.ch/63067/8/1-s2.0-S0304401714003549-main%20Kopie.pdf Gottstein, Bruno; Frey, Caroline; Campbell-Palmer, R; Pizzi, R; Barlow, A; Hentrich, Brigitte; Posautz, A; Ryser-Degiorgis, Marie-Pierre (2014). Immunoblotting for the serodiagnosis of alveolar echinococcosis in alive and dead Eurasian beavers (Castor fiber). Veterinary parasitology, 205(1-2), pp. 113-118. Elsevier 10.1016/j.vetpar.2014.06.017 <http://dx.doi.org/10.1016/j.vetpar.2014.06.017> doi:10.7892/boris.63067 info:doi:10.1016/j.vetpar.2014.06.017 info:pmid:24986466 urn:issn:0304-4017 |
| Idioma(s) |
eng |
| Publicador |
Elsevier |
| Relação |
http://boris.unibe.ch/63067/ |
| Direitos |
info:eu-repo/semantics/restrictedAccess info:eu-repo/semantics/openAccess |
| Fonte |
Gottstein, Bruno; Frey, Caroline; Campbell-Palmer, R; Pizzi, R; Barlow, A; Hentrich, Brigitte; Posautz, A; Ryser-Degiorgis, Marie-Pierre (2014). Immunoblotting for the serodiagnosis of alveolar echinococcosis in alive and dead Eurasian beavers (Castor fiber). Veterinary parasitology, 205(1-2), pp. 113-118. Elsevier 10.1016/j.vetpar.2014.06.017 <http://dx.doi.org/10.1016/j.vetpar.2014.06.017> |
| Palavras-Chave | #630 Agriculture #570 Life sciences; biology #610 Medicine & health |
| Tipo |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion PeerReviewed |
