Lipid-protein interactions drive membrane protein topogenesis in accordance with the positive inside rule.


Autoria(s): Bogdanov, Mikhail; Xie, Jun; Dowhan, William
Data(s)

10/04/2009

Resumo

Transmembrane domain orientation within some membrane proteins is dependent on membrane lipid composition. Initial orientation occurs within the translocon, but final orientation is determined after membrane insertion by interactions within the protein and between lipid headgroups and protein extramembrane domains. Positively and negatively charged amino acids in extramembrane domains represent cytoplasmic retention and membrane translocation forces, respectively, which are determinants of protein orientation. Lipids with no net charge dampen the translocation potential of negative residues working in opposition to cytoplasmic retention of positive residues, thus allowing the functional presence of negative residues in cytoplasmic domains without affecting protein topology.

Identificador

http://digitalcommons.library.tmc.edu/uthmed_docs/111

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665083/?tool=pmcentrez

Publicador

DigitalCommons@The Texas Medical Center

Fonte

UT Medical School Journal Articles

Palavras-Chave #Amino Acids #Biochemistry #Cell Membrane #Cytoplasm #Escherichia coli #Escherichia coli Proteins #Lipid Bilayers #Lipids #Membrane Proteins #Membrane Transport Proteins #Models #Biological #Protein Conformation #Protein Engineering #Protein Transport #Models, Biological #Medicine and Health Sciences
Tipo

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