RMI1 is Essential for Early Embryonic Development and Attenuation of Tumor Development
Data(s) |
01/12/2010
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Resumo |
RMI1 (BLM-Associated Protein 75 or Blap75) is highly conserved from yeast to human. Previous studies have shown that hRMI1 is required for BLM/TopoIIIα/RMI1 complex stability and function. However, in vivo functions of RMI1 remain elusive. To address this question, I generated RMI1 knockout mice by homologous replacement targeting. While RMI1+/- mice showed no obvious phenotype, deletion of both RMI1 alleles leads to early embryonic lethality before implantation. I then generated RMI1/p53 double knockout mice. After ionizing radiation treatment at 4Gy, RMI1/p53 double-heterzygous mice showed shortened tumor latency and aggressive tumor types when comparing with wild type, RMI1+/- and p53+/- control cohorts. My study suggests a dual-functional role of RMI1 in early embryonic development and tumor suppression. |
Formato |
application/pdf |
Identificador |
http://digitalcommons.library.tmc.edu/utgsbs_dissertations/94 http://digitalcommons.library.tmc.edu/cgi/viewcontent.cgi?article=1119&context=utgsbs_dissertations |
Publicador |
DigitalCommons@The Texas Medical Center |
Fonte |
UT GSBS Dissertations and Theses (Open Access) |
Palavras-Chave | #RMI1 #HR #DSB. #Molecular Genetics |
Tipo |
text |