Absolute quantitative real-time polymerase chain reaction for the measurement of human papillomavirus E7 mRNA in cervical cytobrush specimens.
Data(s) |
01/01/2007
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Resumo |
BACKGROUND: Few reports of the utilization of an accurate, cost-effective means for measuring HPV oncogene transcripts have been published. Several papers have reported the use of relative quantitation or more expensive Taqman methods. Here, we report a method of absolute quantitative real-time PCR utilizing SYBR-green fluorescence for the measurement of HPV E7 expression in cervical cytobrush specimens. RESULTS: The construction of a standard curve based on the serial dilution of an E7-containing plasmid was the key for being able to accurately compare measurements between cervical samples. The assay was highly reproducible with an overall coefficient of variation of 10.4%. CONCLUSION: The use of highly reproducible and accurate SYBR-based real-time polymerase chain reaction (PCR) assays instead of performing Taqman-type assays allows low-cost, high-throughput analysis of viral mRNA expression. The development of such assays will help in refining the current screening programs for HPV-related carcinomas. |
Identificador |
http://digitalcommons.library.tmc.edu/uthdb_docs/8 http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1852093&tool=pmcentrez&rendertype=abstract |
Publicador |
DigitalCommons@The Texas Medical Center |
Fonte |
Journal Articles |
Palavras-Chave | #Dentistry |
Tipo |
text |