Bovine Staphylococcus aureus: diagnostic properties of specific media


Autoria(s): Graber, Hans Ulrich; Pfister, S.; Burgener, Pascale; Boss, Renate; Meylan, Mireille; Hummerjohann, J.
Data(s)

01/08/2013

Resumo

As accurate discrimination between Staphylococcus (S.) aureus and NSA (non-S. aureus staphylococci) involved in bovine mastitis is essential in terms of clinical prognosis and outcome, the aim of this study was to reevaluate the classical bacteriological procedures to identify these agents. Various media and the coagulase tube test were investigated using 116 strains of S. aureus and 115 of NSA, all isolated from cows with spontaneous intramammary infections (IMI). Furthermore, 25 NSA reference strains were analyzed. The study demonstrated that a few media were appropriate for differentiating S. aureus from NSA, provided that the staphylococci were isolated from bovine IMI. Evaluation of hemolysis further revealed that double or incomplete hemolysis are specific for S. aureus and are, therefore, a decisive diagnostic criterion. For strains showing complete hemolysis, maximal discrimination between S. aureus and NSA was observed by subculturing them on CHROMagar Staph. aureus.

Formato

application/pdf

Identificador

http://boris.unibe.ch/45817/1/1-s2.0-S0034528813000775-main.pdf

Graber, Hans Ulrich; Pfister, S.; Burgener, Pascale; Boss, Renate; Meylan, Mireille; Hummerjohann, J. (2013). Bovine Staphylococcus aureus: diagnostic properties of specific media. Research in veterinary science, 95(1), pp. 38-44. Association for Veterinary Teaching and Research Work 10.1016/j.rvsc.2013.02.023 <http://dx.doi.org/10.1016/j.rvsc.2013.02.023>

doi:10.7892/boris.45817

info:doi:10.1016/j.rvsc.2013.02.023

info:pmid:23548479

urn:issn:0034-5288

Idioma(s)

eng

Publicador

Association for Veterinary Teaching and Research Work

Relação

http://boris.unibe.ch/45817/

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Graber, Hans Ulrich; Pfister, S.; Burgener, Pascale; Boss, Renate; Meylan, Mireille; Hummerjohann, J. (2013). Bovine Staphylococcus aureus: diagnostic properties of specific media. Research in veterinary science, 95(1), pp. 38-44. Association for Veterinary Teaching and Research Work 10.1016/j.rvsc.2013.02.023 <http://dx.doi.org/10.1016/j.rvsc.2013.02.023>

Palavras-Chave #630 Agriculture
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

PeerReviewed