Domain analysis of lipoprotein LppQ in Mycoplasma mycoides subsp. mycoides SC


Autoria(s): Bonvin, Laetitia Anne; Vilei, Edy M.; Kühni Boghenbor, Kathrin; Kapp, Nadine; Frey, Joachim; Stoffel, Michael Hubert
Data(s)

2008

Resumo

The lipoprotein LppQ is the most prominent antigen of Mycoplasma mycoides subsp. mycoides small colony type (SC) during infection of cattle. This pathogen causes contagious bovine pleuropneumonia (CBPP), a devastating disease of considerable socio-economic importance in many countries worldwide. The dominant antigenicity and high specificity for M. mycoides subsp. mycoides SC of lipoprotein LppQ have been exploited for serological diagnosis and for epidemiological investigations of CBPP. Scanning electron microscopy and immunogold labelling were used to provide ultrastructural evidence that LppQ is located to the cell membrane at the outer surface of M. mycoides subsp. mycoides SC. The selectivity and specificity of this method were demonstrated through discriminating localization of extracellular (i.e., in the zone of contact with host cells) vs. integral membrane domains of LppQ. Thus, our findings support the suggestion that the accessible N-terminal domain of LppQ is surface exposed and such surface localization may be implicated in the pathogenesis of CBPP.

Formato

application/pdf

Identificador

http://boris.unibe.ch/37769/1/domain_analysis.pdf

Bonvin, Laetitia Anne; Vilei, Edy M.; Kühni Boghenbor, Kathrin; Kapp, Nadine; Frey, Joachim; Stoffel, Michael Hubert (2008). Domain analysis of lipoprotein LppQ in Mycoplasma mycoides subsp. mycoides SC. Antonie van Leeuwenhoek - international journal of general and molecular microbiology, 93(1-2), pp. 175-183. Dordrecht: Springer 10.1007/s10482-007-9191-1 <http://dx.doi.org/10.1007/s10482-007-9191-1>

doi:10.7892/boris.37769

info:doi:10.1007/s10482-007-9191-1

urn:issn:0003-6072

Idioma(s)

eng

Publicador

Springer

Relação

http://boris.unibe.ch/37769/

Direitos

info:eu-repo/semantics/openAccess

Fonte

Bonvin, Laetitia Anne; Vilei, Edy M.; Kühni Boghenbor, Kathrin; Kapp, Nadine; Frey, Joachim; Stoffel, Michael Hubert (2008). Domain analysis of lipoprotein LppQ in Mycoplasma mycoides subsp. mycoides SC. Antonie van Leeuwenhoek - international journal of general and molecular microbiology, 93(1-2), pp. 175-183. Dordrecht: Springer 10.1007/s10482-007-9191-1 <http://dx.doi.org/10.1007/s10482-007-9191-1>

Palavras-Chave #630 Agriculture #570 Life sciences; biology
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

PeerReviewed