THE ROLE OF BROAD IN DETERMINING NEURONAL COMPOSITION IN THE DROSOPHILA BRAIN


Autoria(s): Dincer, Alper
Data(s)

01/01/2011

Resumo

To elucidate the individual roles of the four Broad-Complex (BR-C) isoforms, Z1-Z4, on neuronal composition in the mushroom body, I undertook a series of overexpression experiments and created tools for knockdown experiments. Specifically, I imaged and analyzed Drosophila brains from earlier experiments in which BR-C isoforms Z1 and Z3 were individually overexpressed in the MB. The knockdown experiments required the creation of the molecular tools necessary for isoform-specific RNA interference (RNAi). For these I performed PCR to amplify DNA sequences unique to each isoform and inserted those into the pWIZ vector, which will permit expression of loopless hairpin double stranded RNA to trigger the RNAi pathway in the fly.

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application/pdf

Identificador

http://digitalcommons.bucknell.edu/honors_theses/50

http://digitalcommons.bucknell.edu/cgi/viewcontent.cgi?article=1049&context=honors_theses

Publicador

Bucknell Digital Commons

Fonte

Honors Theses

Palavras-Chave #Drosophila #Mushroom Body #Neuronal Plasticity #Broad-Complex #Developmental Plasticity
Tipo

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