Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees
Data(s) |
01/08/1991
|
---|---|
Resumo |
The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 900. This bend results almost entirely from two 400 kinks that occur between TG/CA base pairs at positions 5 and 6 on each side of the dyad axis of the complex. DNA sequence discrimination by CAP derives both from sequence-dependent distortion of the DNA helix and from direct hydrogen-bonding interactions between three protein side chains and the exposed edges of three base pairs in the major groove of the DNA. The structure of this transcription factor-DNA complex provides insights into possible mechanisms of transcription activation |
Identificador | |
Publicador |
Bucknell Digital Commons |
Fonte |
Faculty Journal Articles |
Palavras-Chave | #Molecular Biology |
Tipo |
text |