Effects of ischemia and reperfusion on subpopulations of rat enteric neurons expressing the P2X7 receptor


Autoria(s): Palombit, Kelly; Mendes, Cristina Eusébio; Lima, Wothan Tavares de; Silveira, Mariana Póvoa; Castelucci, Patricia
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

10/04/2014

10/04/2014

01/12/2013

Resumo

BACKGROUND: Intestinal ischemia followed by reperfusion (I/R) may occur following intestinal obstruction. In rats, I/R in the small intestine leads to structural changes accompanied by neuronal death. AIM: To analyze the impact of I/R injury on different neuronal populations in the myenteric plexus of rat ileum. METHODS: The ileal artery was occluded for 35 min and animals were euthanized 6, 24, and 72 h, and 1 week later. Immunohistochemistry was performed with antibodies against the P2X7 receptor as well as nitric oxide synthase (NOS), calbindin, calretinin, choline acetyltransferase (ChAT), or the pan-neuronal marker anti-HuC/D. RESULTS: Double immunolabeling demonstrated that 100% of NOS-, calbindin-, calretinin-, and ChAT-immunoreactive neurons in all groups expressed the P2X7 receptor. Following I/R, neuronal density decreased by 22.6% in P2X7 receptor-immunoreactive neurons, and decreased by 46.7, 38, 39.8, 21.7, and 20% in NOS-, calbindin-, calretinin-, ChAT-, and HuC/D-immunoreactive neurons, respectively, at 6, 24, and 72 h and 1 week following injury compared to the control and sham groups. We also observed a 14% increase in the neuronal cell body profile area of the NOS-immunoreactive neurons at 6 and 24 h post-I/R and a 14% increase in ChAT-immunoreactive neurons at 1 week following I/R. However, the average size of the calretinin-immunoreactive neurons was reduced by 12% at 6 h post-I/R and increased by 8% at 24 h post-I/R. CONCLUSIONS: This work demonstrates that I/R is associated with a significant loss of different subpopulations of neurons in the myenteric plexus accompanied by morphological changes, all of which may underlie conditions related to intestinal motility disorder

FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo, 08/05314-5)

CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior)

Identificador

Digestive Diseases and Sciences, New York, v.58, n.12, p.3429-3439, 2013

http://www.producao.usp.br/handle/BDPI/44453

doi: 10.1007/s10620-013-2847-y

http://dx.doi.org/10.1007/s10620-013-2847-y

Idioma(s)

eng

Publicador

Springer Science+Business Media

New York

Relação

Digestive Diseases and Sciences

Direitos

restrictedAccess

Springer Science+Business Media

Palavras-Chave #Chemical coding #Myenteric neurons #Ischemia and reperfusion #P2X7 receptor #CODIFICAÇÃO #QUÍMICA #NEURÔNIOS #ISQUEMIA #INTESTINO DELGADO
Tipo

article

original article

publishedVersion