How low-level laser therapy can change mechanical properties of cells.


Autoria(s): Magalhães, Ana Carolina de; Muñoz, Diana Maria Martinez; Ferreira, Marcia Z. J.; Chavantes, Maria Cristina; Yoshimura, Elisabeth Mateus; Alencar, Adriano Mesquita
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

25/04/2014

25/04/2014

02/02/2013

Resumo

Low level laser therapy is used as a treatment of several conditions, including inflammatory processes and wound healing. Possible changes in mechanical properties of cells, caused by illumination, are investigated with optical magnetic twisting cytometry (OMTC), which is a technique used to evaluate mechanical properties in cell culture. Ferromagnetic micro beads are bound to cell cytoskeleton, the beads are magnetized vertically and a horizontal twisting magnetic field is applied causing a torque that moves the beads and deforms the cell, the beads rotate and displace. Based on the lateral displacement of the beads, elastic shear and loss moduli are obtained. Samples of human bronchial epithelial cell culture were divided in two groups: one was illuminated with a 660 nm red laser, 30 mW power, 0.75 W/cm2 irradiance, during different time intervals, and the other one, the control group, was not illuminated. The values of the mechanical constants of the cells of the control group showed a tendency of increasing with the time out of the incubator. On the other hand, the illuminated group showed constancy on the behavior of both moduli, keeping the normal conditions of the cell culture. Those results indicate that illumination can induce cells to homeostasis, and OMTC is sensitive to observe departures from the steady conditions. Hence, OMTC is an important technique which can be used to aggregate knowledge on the light effect in cell cytoskeleton and even on the low level laser therapy mechanisms in inflammatory processes and/or wound healing.

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Identificador

Mechanisms for Low-Light Therapy, VIII, 2013, San Francisco.

0819493384

http://www.producao.usp.br/handle/BDPI/44650

http://proceedings.spiedigitallibrary.org/data/Conferences/SPIEP/73167/856909.pdf

Idioma(s)

eng

Publicador

The Moscone Center

San Francisco

Relação

Mechanisms for Low-Light Therapy, VIII

Direitos

restrictedAccess

COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE)

Palavras-Chave #Optical magnetic twisting cytometry #Cell mechanical properties #Low level laser therapy #Terapia a laser #Citoesqueleto #Células epiteliais
Tipo

conferenceObject

Resumo