IgG Antibody responses in mice coinfected with Toxocara canis and other helminths or protozoan parasites
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
---|---|
Data(s) |
04/11/2013
04/11/2013
2012
|
Resumo |
The immune response expressed by IgG antibodies in BALB/c mice experimentally infected with Toxocara canis, was studied with the aim of verifying the possible in vivo cross-reactivity between antigens of T. canis and other parasites (Ascaris suum, Taenia crassiceps, Schistosoma mansoni, Strongyloides venezuelensis and Toxoplasma gondii). Experiments included three groups of mice: one infected only by T. canis, another with one of the other species of parasites and a third concomitantly infected with T. canis and the other species in question. Animals were bled by orbital plexus at 23, 38 and 70 days post infection (p.i.). Sera were analyzed for anti-Toxocara antibodies by ELISA and Immunoblotting, using excretion-secretion antigens (ES), obtained from culture of third-stage larvae of T. canis. For all experiments a control group comprised by ten non-infected mice was used. Only in the case of A. suum infection, in these experimental conditions, the occurrence of cross-reactivity with T. canis was observed. However, in the case of co-infection of T. canis - S. mansoni, T. canis - S. venezuelensis and T. canis - T. crassiceps the production of anti-Toxocara antibodies was found at levels significantly lower than those found in mice infected with T. canis only. Co-infection with S. mansoni or S. venezuelensis showed lower mortality rates compared to what occurred in the animals with single infections. Results obtained in mice infected with T. canis and T. gondii showed significant differences between the mean levels of the optical densities of animals infected with T. canis and concomitantly infected with the protozoan only in the 23rd day p.i. |
Identificador |
Rev. Inst. Med. trop. S. Paulo,v.54,n.3,p.145-152,2012 0036-4665 http://www.producao.usp.br/handle/BDPI/38280 10.1590/S0036-46652012000300006 http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0036-46652012000300006&lng=en&nrm=iso&tlng=en |
Idioma(s) |
eng |
Publicador |
Instituto de Medicina Tropical |
Relação |
Revista do Instituto de Medicina Tropical de São Paulo |
Direitos |
openAccess |
Palavras-Chave | #Toxocara canis #Ascaris suum #Taenia crassiceps #Schistosoma mansoni #Strongyloides venezuelensis #Toxoplasma gondii #Coinfection #IgG #Humoral response |
Tipo |
article original article |