Evaluation of GBV-C / HVG viremia in HIV-infected women


Autoria(s): Silva, Synara Araújo; Rodrigues, Célia Lima; Campos, Aléia Faustino; Levi, José Eduardo
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

04/11/2013

04/11/2013

2012

Resumo

The present study aimed at standardizing a real-time quantitative polymerase chain reaction assay to evaluate the presence of GBV-C/HGV RNA. A "TaqMan" assay using primers and probe derived from the 5¢ NCR region was developed and validated. Two hundred and fifty-three plasma samples from HIV-infected women were tested for GBV-C viremia and antibody against the envelope protein 2. GBV-C RNA was detected in 22.5% of the patients whereas the antibody was identified in 25.3% of the cohort. Detection of viral RNA and of antibodies was mutually exclusive. Viral loads showed a mean of 1,777 arbitrary units / mL, being 1.1 and 13,625 arbitrary units / mL respectively the lowest and highest values measured. We conclude that the real-time quantitative polymerase chain reaction method developed is appropriate for the investigation of GBV-C RNA since it was shown to be highly specific and sensitive, as well as requiring few steps, preventing contamination and providing additional information as to the relative viremia of carriers, a parameter that must be included in studies evaluating the co-factors influencing the clinical outcome of HIV/AIDS.

Identificador

Rev. Inst. Med. trop. S. Paulo,v.54,n.1,p.31-35,2012

0036-4665

http://www.producao.usp.br/handle/BDPI/38275

10.1590/S0036-46652012000100006

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652012000100006&lng=en&nrm=iso&tlng=en

http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0036-46652012000100006&lng=en&nrm=iso&tlng=en

http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0036-46652012000100006&lng=en&nrm=iso&tlng=en

Idioma(s)

eng

Publicador

Instituto de Medicina Tropical

Relação

Revista do Instituto de Medicina Tropical de São Paulo

Direitos

openAccess

Palavras-Chave #GBV-C #Real-Time PCR #Viral load #Anti-HGenv #AIDS
Tipo

article

original article