Invasion of Ureaplasma diversum in bovine spermatozoids
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
|---|---|
| Data(s) |
14/10/2013
14/10/2013
2011
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| Resumo |
Background Ureaplasma diversum has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze in vitro interaction of U. diversum isolates and ATCC strains with bovine spermatozoids. The interactions were observed by confocal microscopy and the gentamycin internalization assay. Findings U. diversum were able to adhere to and invade spermatozoids after 30 min of infection. The gentamicin resistance assay confirmed the intracellularity and survival of U. diversum in bovine spermatozoids. Conclusions The intracellular nature of bovine ureaplasma identifies a new difficulty to control the reproductive of these animals. This study was supported by FAPESP (grant 06/56855-0). We thank Aricelma P. França for invaluable technical assistance and AcademicEnglishSolutions.com for revising the English. |
| Identificador |
BMC Research Notes, London, v.4, p.1-8, 2011 1756-0500 http://www.producao.usp.br/handle/BDPI/34955 10.1186/1756-0500-4-455 |
| Idioma(s) |
eng |
| Publicador |
BioMed Central London |
| Relação |
BMC Research Notes |
| Direitos |
openAccess Timenetsky et al; licensee BioMed Central Ltd. - This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Palavras-Chave | #Ureaplasma diversum #bovine spermatozoid #invasion #confocal microscopy |
| Tipo |
article original article publishedVersion |
