VEGF-C expression in oral cancer by neurotransmitter-induced activation of beta-adrenergic receptors
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
16/10/2013
16/10/2013
01/02/2013
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Resumo |
The aim of this study was to investigate the expression of vascular endothelial growth factor type C (VEGF-C) in oral squamous cell carcinoma (OSCC) cell lines through norepinephrine-induced activation of beta-adrenergic receptors. Human OSCC cell lines (SCC-9 and SCC-25) expressing beta-adrenergic receptors were stimulated with different concentrations of norepinephrine (0.1, 1, and 10 μM) and 1 μMof propranolol, and analyzed after 1, 6, and 24 h. VEGF-C gene expression and VEGF-C production in the cell supernatant were evaluated by real-time PCR and by ELISA, respectively. The results showed that beta-adrenergic receptor stimulation by different concentrations of norepinephrine or blocking by propranolol did not markedly alter VEGF-C expression by SCC-9 and SCC-25 cells. VEGF-C protein levels produced by oral malignant cell lines after stimulation with different norepinephrine concentrations or blocking with propranolol was statistically similar (p>0.05) to those of the control group (nonstimulated OSCC cell lines). Our findings suggest that stimulation of beta-adrenergic receptors by means of norepinephrine does not seem to modulate the VEGF-C expression in OSCC cell lines. These findings reinforce the need for further studies in order to understand the responsiveness of oral cancer to beta-adrenergic receptor stimulation or blockage, especially with regard to VEGF-C production. CNPq 476139/2010-4, 500991/2010-3 FAPESP 2010/06333-3, 2010/51361-5 |
Identificador |
Tumor Biology, Basel, v. 34, n. 1, p. 139-143, Feb. 2013 1010-4283 http://www.producao.usp.br/handle/BDPI/35158 10.1007/s13277-012-0521-1 http://link.springer.com/content/pdf/10.1007%2Fs13277-012-0521-1.pdf |
Idioma(s) |
eng |
Publicador |
Springer Basel |
Relação |
Tumor Biology |
Direitos |
restrictedAccess Springer |
Palavras-Chave | #Oral cancer #VEGF #Adrenergic receptors #NEOPLASIAS #BOCA #RECEPTORES ADRENÉRGICOS |
Tipo |
article original article publishedVersion |