Comparative analysis of Enterococcus Faecalis biofilm formation on different substrates
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
---|---|
Data(s) |
11/10/2013
11/10/2013
01/03/2013
|
Resumo |
Introduction: The aim of this study was to compare Enterococcus faecalis biofilm formation on different substrates. Methods: Cell culture plates containing growth medium and E. faecalis (ATCC 29212) were used to grow biofilm on bovine dentin, gutta-percha, hydroxyapatite, or bovine bone. Substrates were incubated at 37 C for 14 or 21 days, and the medium was changed every 48 hours. After the growth induction periods, specimens (n = 5 per group and per induction period) were stained by using Live/Dead, and the images were analyzed under a confocal microscope. The total biovolume (mm3), live bacteria biovolume (mm3), and substrate coverage (%) were quantified by using the BioImage_L software. Results obtained were analyzed by nonparametric tests (P = .05). Results: Biofilm formation was observed in all groups. Gutta-percha had the lowest total biovolume at 14 days (P < .05) and hydroxyapatite the highest at 21 days (P < .05). No significant difference was observed in green biovolume at 14 days. At 21 days, however, hydroxyapatite had the highest volume (P < .05). The percentages of coverage were similar among all substrates at 21 days (P > .05), but at 14 days, bovine bone presented the highest coverage (P < .05). Conclusions: E. faecalis was capable of forming biofilm on all substrates during both growth periods; hydroxyapatite presented the highest rates of biofilm formation. The type of substrate influenced the biofilm characteristics, according to the parameters evaluated |
Identificador |
Journal of Endodontics, Chicago, v. 39, n. 3, p. 346-350, Mar. 2013 0099-2399 http://www.producao.usp.br/handle/BDPI/34132 10.1016/j.joen.2012.09.027 |
Idioma(s) |
eng |
Publicador |
Elsevier Chicago |
Relação |
Journal of Endodontics |
Direitos |
restrictedAccess Elsevier |
Palavras-Chave | #Biofilm #Confocal microscopy #Enterococcus faecalis #BIOFILMES #MICROSCOPIA CONFOCAL #ENTEROCOCCUS |
Tipo |
article original article publishedVersion |