Collybistin and gephyrin are novel components of the eukaryotic translation initiation factor 3 complex
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
26/08/2013
26/08/2013
2010
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Resumo |
Abstract Background Collybistin (CB), a neuron-specific guanine nucleotide exchange factor, has been implicated in targeting gephyrin-GABAA receptors clusters to inhibitory postsynaptic sites. However, little is known about additional CB partners and functions. Findings Here, we identified the p40 subunit of the eukaryotic translation initiation factor 3 (eIF3H) as a novel binding partner of CB, documenting the interaction in yeast, non-neuronal cell lines, and the brain. In addition, we demonstrated that gephyrin also interacts with eIF3H in non-neuronal cells and forms a complex with eIF3 in the brain. Conclusions Together, our results suggest, for the first time, that CB and gephyrin associate with the translation initiation machinery, and lend further support to the previous evidence that gephyrin may act as a regulator of synaptic protein synthesis. We thank Dr. Beatriz A. Castilho, Martin Roffe, Dr. Luis Soares Netto and Dr. Gisele Monteiro for fruitful discussions and advising on the experiments. We thank Constância G. Urbani for secretarial assistance. This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). We thank Dr. Beatriz A. Castilho, Martin Roffe, Dr. Luis Soares Netto and Dr. Gisele Monteiro for fruitful discussions and advising on the experiments. We thank Constância G. Urbani for secretarial assistance. This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). |
Identificador |
1756-0500 http://www.producao.usp.br/handle/BDPI/33166 10.1186/1756-0500-3-242 |
Idioma(s) |
eng |
Relação |
BMC Research Notes |
Direitos |
openAccess Sertie et al; licensee BioMed Central Ltd. - This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Tipo |
article original article |