Effects of myenteric denervation on extracellular matrix fibers and mast cell distribution in normal stomach and gastric lesions


Autoria(s): Estofolete, Cássia F; Botelho-Machado, Carla ; Taboga, Sebastião R; Zucoloto, Sérgio ; Polli-Lopes, Ana ; Gil, Cristiane D
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

26/08/2013

26/08/2013

01/06/2010

Resumo

Abstract Background In this study the effect of myenteric denervation induced by benzalconium chloride (BAC) on distribution of fibrillar components of extracellular matrix (ECM) and inflammatory cells was investigated in gastric carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Rats were divided in four experimental groups: non-denervated (I) and denervated stomach (II) without MNNG treatment; non-denervated (III) and denervated stomachs (IV) treated with MNNG. For histopathological, histochemical and stereological analysis, sections of gastric fragments were stained with Hematoxylin-Eosin, Picrosirius-Hematoxylin, Gomori reticulin, Weigert's Resorcin-Fuchsin, Toluidine Blue and Alcian-Blue/Safranin (AB-SAF). Results BAC denervation causes an increase in the frequency of reticular and elastic fibers in the denervated (group II) compared to the non-denervated stomachs (group I). The treatment of the animals with MNNG induced the development of adenocarcinomas in non-denervated and denervated stomachs (groups III and IV, respectively) with a notable increase in the relative volume of the stroma, the frequency of reticular fibers and the inflammatory infiltrate that was more intense in group IV. An increase in the frequency of elastic fibers was observed in adenocarcinomas of denervated (group IV) compared to the non-denervated stomachs (group III) that showed degradation of these fibers. The development of lesions (groups III and IV) was also associated with an increase in the mast cell population, especially AB and AB-SAF positives, the latter mainly in the denervated group IV. Conclusions The results show a strong association in the morphological alteration of the ECM fibrillar components, the increased density of mast cells and the development of tumors induced by MNNG in the non-denervated rat stomach or denervated by BAC. This suggests that the study of extracellular and intracellular components of tumor microenvironment contributes to understanding of tumor biology by action of myenteric denervation.

We are grateful to Domingos Zanchetta Netto and Luiz Roberto Falleiros-Jr for technical assistance. CFE and CBM were supported by Fundação de Amparo á Pesquisa - FAPESP (grants 08/05722-6 and 03/10634-5, respectively) and SRT by Conselho Nacional de Desenvolvimento Cientifico e Tecnológico - CNPq (grants 301111/05-7 and 300163/2008-8).

We are grateful to Domingos Zanchetta Netto and Luiz Roberto FalleirosJr for technical assistance. CFE and CBM were supported by Fundação de Amparo á Pesquisa FAPESP (grants 08/057226 and 03/106345, respectively) and SRT by Conselho Nacional de Desenvolvimento Cientifico e Tecnológico CNPq (grants 301111/057 and 300163/20088).

Identificador

1475-2867

http://www.producao.usp.br/handle/BDPI/32945

10.1186/1475-2867-10-18

http://www.cancerci.com/content/10/1/18

Idioma(s)

eng

Relação

Cancer Cell International

Direitos

openAccess

Estofolete et al; licensee BioMed Central Ltd. - This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Tipo

article

original article