Time course of hydrogen peroxide-thioredoxin balance and its influence on the intracellular signalling in myocardial infarction
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
07/11/2013
07/11/2013
2012
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Resumo |
We investigated the myocardial thioredoxin-1 and hydrogen peroxide concentrations and their association with some prosurvival and pro-apoptotic proteins, during the transition from myocardial infarction (MI) to heart failure in rats. Male Wistar rats were divided into the following six groups: three sham-operated groups and three MI groups, each at at 2, 7 and 28 days postsurgery. Cardiac function was analysed by echocardiography; the concentration of H2O2 and the ratio of reduced to oxidized glutathione were measured spectrophotometrically, while the myocardial immunocontent of thioredoxin-1, angiotensin II, angiotensin II type 1 and type 2 receptors, p-JNK/JNK, p-ERK/ERK, p-Akt/Akt, p-mTOR/mTOR and p-GSK3 beta/GSK3 beta was evaluated by Western blot. Our results show that thioredoxin-1 appears to make an important contribution to the reduced H2O2 concentration. It was associated with lower JNK expression in the early period post-MI (2 days). However, thioredoxin-1 decreased, while reninangiotensin system markers and levels of H2O2 increased, over 28 days post-MI, in parallel with some signalling proteins involved in maladaptative cardiac remodelling and ventricular dysfunction. These findings provide insight into the time course profile of endogenous antioxidant adaptation to ischaemic injury, which may be useful for the design of therapeutical strategies targeting oxidative stress post-MI. Secretaria de Ciencia e Tecnologia do Estado do Rio Grande do Sul Secretaria de Ciencia e Tecnologia do Estado do Rio Grande do Sul CNPq CNPq CAPES CAPES FAPERGS FAPERGS |
Identificador |
EXPERIMENTAL PHYSIOLOGY, HOBOKEN, v. 97, n. 6, supl. 1, Part 1, pp. 741-749, JUN, 2012 0958-0670 http://www.producao.usp.br/handle/BDPI/43237 10.1113/expphysiol.2012.064832 |
Idioma(s) |
eng |
Publicador |
WILEY-BLACKWELL HOBOKEN |
Relação |
EXPERIMENTAL PHYSIOLOGY |
Direitos |
closedAccess Copyright WILEY-BLACKWELL |
Palavras-Chave | #OXIDATIVE STRESS #HEART-FAILURE #RATS #PATHWAYS #ANTIOXIDANT #ACTIVATION #AKT #PHYSIOLOGY |
Tipo |
article original article publishedVersion |