Human bocavirus infection diagnosed serologically among children admitted to hospital with community-acquired pneumonia in a tropical region
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
06/11/2013
06/11/2013
2012
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Resumo |
Human bocavirus (HBoV) is a human virus associated with respiratory disease in children. Limited information is available on acute infection with HBoV among children admitted to hospital with community-acquired pneumonia in tropical regions and the current diagnosis is inadequate. The aims were to diagnose and describe acute HBoV infections among children hospitalized for community-acquired pneumonia. In Salvador, Brazil, 277 children with community-acquired pneumonia were prospectively enrolled. Paired serum samples were tested by IgG, IgM, and IgG-avidity enzyme immunoassays (EIAs) using recombinant HBoV VP2. HBoV DNA was detected in nasopharyngeal aspirates and serum by a quantitative polymerase-chain reaction (PCR). HBoV DNA was detected in nasopharyngeal aspirates of 62/268 (23%) children and 156/273 (57%) were seropositive. Acute primary HBoV infection was reliably diagnosed (bearing at least two acute markers: Positive IgM, a fourfold increase/conversion of IgG, low IgG avidity or viremia) in 21 (8%) of 273 patients, 90% of 20 had HBoV DNA in nasopharyngeal aspirates, 83% with a high DNA load. The median age of infection with HBoV was 16 months, range 5-36.Community-acquired pneumonia was confirmed radiographically in 85% of 20 patients with acute HBoV infection diagnosed serologically. HBoV DNA was found in nasopharyngeal aspirates of 42/246(17%) children without an acute primary HBoV infection and available nasopharyngeal aspirate. Four children with HBoV secondary immune responses were detected, lacking both IgM and viremia. HBoV infection was diagnosed accurately in children aged 5-36 months with community-acquired pneumonia confirmed radiographically. PCR of nasopharyngeal aspirates is not a reliable marker of acute HBoV infection. J. Med. Virol. 84:253-258, 2012. (C) 2011 Wiley Periodicals, Inc. Fundacao de Amparo a Pesquisa no Estado da Bahia (FAPESB), Salvador, Brazil Fundacao de Amparo a Pesquisa no Estado da Bahia (FAPESB), Salvador, Brazil Academy of Finland [1122539] Academy of Finland Medical Society of Finland Medical Society of Finland University of Helsinki University of Helsinki Sigrid Juselius Foundation, Helsinki, Finland Sigrid Juselius Foundation, Helsinki, Finland |
Identificador |
JOURNAL OF MEDICAL VIROLOGY, MALDEN, v. 84, n. 2, supl. 1, Part 1, pp. 253-258, FEB, 2012 0146-6615 http://www.producao.usp.br/handle/BDPI/42444 10.1002/jmv.22268 |
Idioma(s) |
eng |
Publicador |
WILEY-BLACKWELL MALDEN |
Relação |
JOURNAL OF MEDICAL VIROLOGY |
Direitos |
closedAccess Copyright WILEY-BLACKWELL |
Palavras-Chave | #ACUTE RESPIRATORY INFECTION #LOWER RESPIRATORY TRACT INFECTION #RESPIRATORY VIRUS #RESPIRATORY TRACT INFECTION #RESPIRATORY-TRACT INFECTIONS #PARVOVIRUS #INFANTS #VIROLOGY |
Tipo |
article original article publishedVersion |