Evaluation of the schistosomicidal activity of the steroidal alkaloids from Solanum lycocarpum fruits


Autoria(s): Miranda, Mariza Abreu; Magalhaes, Lizandra Guidi; Jorge Tiossi, Renata Fabiane; Kuehn, Christian Collins; Rodrigues Oliveira, Luiz Gustavo; Rodrigues, Vanderlei; McChesney, James Dewey; Bastos, Jairo Kenupp
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

05/11/2013

05/11/2013

2012

Resumo

Solanum lycocarpum (Solanaceae), a Brazilian medicinal plant known as "wolf fruit," contains about 1.5% of glycoalkaloids in its dried fruits, consisting mainly of solamargine and solasonine. The present work reports the obtainment of the alkaloidic extract of the S. lycocarpum fruit by acid-base extraction and the isolation of the major alkaloid heterosides by chromatographic means, as well as the evaluation of their in vitro schistosomicidal activities. The in vitro schistosomicidal activities of the alkaloidic extract of S. lycocarpum fruits and its isolated steroidal alkaloids were undertaken against adult worms of Schistosoma mansoni. The alkaloidic extract (20, 32, and 50 mu g mL(-1)), solasonine (50 mu M), solamargine (32 and 50 mu M), and equimolar mixture of glycoalkaloids (20, 32, and 50 mu M) lead to the separation of all couple worms and extensive disruption on their teguments, such as sloughing, as well as their deaths within 24 h of incubation. In addition, the alkaloidic extract (10 and 15 mu g mL(-1)), solasonine (50 mu M), solamargine (10, 15, and 20 mu M), and equimolar mixtures of glycoalkaloids (10 and 15 mu M) reduced the development of eggs produced by the adult worms. Solamargine, containing the sugar chain moiety chacotriose, was more active than the solasonine, which contains solatriose sugar chain moiety. A synergistic effect was also observed for a mixture of solamargine and solasonine. Therefore, the alkaloidic extract of S. lycocarpum, and its major components, solamargine and solasonine, showed promising schistosomicidal activity.

FAPESP [2008/56324-0]

FAPESP

Identificador

PARASITOLOGY RESEARCH, NEW YORK, v. 111, n. 1, supl. 1, Part 1, pp. 257-262, JUL, 2012

0932-0113

http://www.producao.usp.br/handle/BDPI/41845

10.1007/s00436-012-2827-8

http://dx.doi.org/10.1007/s00436-012-2827-8

Idioma(s)

eng

Publicador

SPRINGER

NEW YORK

Relação

PARASITOLOGY RESEARCH

Direitos

closedAccess

Copyright SPRINGER

Palavras-Chave #IN-VITRO #ADULT WORMS #MANSONI #GLYCOALKALOIDS #INHIBITION #PRAZIQUANTEL #SOLAMARGINE #SOLASONINE #GLYCOSIDES #EFFICACY #PARASITOLOGY
Tipo

article

original article

publishedVersion