A SURVEY OF NEOSPORA CANINUM AND TOXOPLASMA GONDII INFECTION IN URBAN RODENTS FROM BRAZIL


Autoria(s): Muradian, Vanessa; Ferreira, Leandra Ribeiro; Lopes, Estela Gallucci; Esmerini, Patrícia de Oliveira; Pena, Hilda Fatima de Jesus; Soares, Rodrigo Martins; Gennari, Solange Maria
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

07/11/2013

07/11/2013

2012

Resumo

Toxoplasma gondii is a protozoan parasite that infects humans and other warm-blooded animals; it uses feral and domestic cats as the definitive hosts. Neospora caninum is a protozoan parasite of animals whose life cycle is very similar to T. gondii but uses canids as definitive hosts. Small rodents play an important role in the life cycle of T. gondii, and a few findings indicated that they may be natural intermediate hosts for N. caninum. The present study was aimed at identifying infections by T. gondii and N. caninum in urban rodents. Infections by T. gondii were quantified using isolation of the parasite by bioassay in mice; molecular methods were also used for both parasites. Overall, 217 rodents were captured. Brain and heart tissues of all rodents were bioassayed in mice for the detection of T. gondii infection. Brain and heart tissues of 121 rodents had the DNA extracted for molecular analysis. Toxoplasma gondii was isolated by bioassay from a single rodent. From the 121 rodents tested for the presence of T. gondii DNA, 2 animals were positive. In contrast, DNA of N. caninum was not detected in any of the samples. In conclusion, the surveys of N. caninum and T. gondii infection in Rattus rattus, Rattus norvegicus, and Mus musculus captured in urban areas of Sao Paulo reveal a striking low frequency of occurrence of these infections.

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [04/12854-5]

Identificador

JOURNAL OF PARASITOLOGY, LAWRENCE, v. 98, n. 1, pp. 128-134, FEB, 2012

0022-3395

http://www.producao.usp.br/handle/BDPI/42983

10.1645/GE-2817.1

http://dx.doi.org/10.1645/GE-2817.1

Idioma(s)

eng

Publicador

AMER SOC PARASITOLOGISTS

LAWRENCE

Relação

JOURNAL OF PARASITOLOGY

Direitos

closedAccess

Copyright AMER SOC PARASITOLOGISTS

Palavras-Chave #SAO-PAULO STATE #HAMMONDIA-HEYDORNI #RATTUS-NORVEGICUS #DEFINITIVE HOSTS #PCR ANALYSIS #OOCYSTS #TISSUES #CATS #SEROPREVALENCE #MICE #PARASITOLOGY
Tipo

article

original article

publishedVersion