Evaluation of Inductively Coupled Plasma Mass Spectrometry for Determining Ca, Cu, Fe, Mg, Mn, Se and Zn in Bovine Semen Samples using a Simple Sample Dilution Method


Autoria(s): Aguiar, Giovanna de Fatima Moreno; Batista, Bruno Lemos; Rodrigues, Jairo Lisboa; Luccas, Pedro O.; Junior, Fernando Barbosa
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

07/11/2013

07/11/2013

2012

Resumo

A simple and fast method for the determination of Ca, Cu, Fe, Mg, Mn, Se and Zn in bovine semen by quadrupole inductively coupled plasma spectrometry (q-ICP-MS) is described. Prior to analysis, samples (200 mu L) were diluted 1:50 in a solution containing 0.01% v/v Triton (R) X-100 and 0.5% v/v nitric acid and directly analyzed by ICP-MS. The limits of detection of the method are 0.3, 0.03, 0.2, 0.04, 0.04, 0.03 and 0.03 mu g L-1 for Ca-44, Cu-63, Fe-57, Mg-24, Zn-64, Se-82 and Mn-55, respectively. For purposes of comparison and method validation, four ordinary bovine semen samples were directly analyzed by ICP-MS and by flame atomic absorption spectrometry (FAAS) or graphite furnace atomic absorption spectrometry (GF AAS), with no statistical difference between the techniques at the 95% level when applying the t-test. Then, the proposed method was applied in the determinations of Ca, Cu, Fe, Mg, Mn, Se and Zn in collected samples of bovine semen from different breeds, which are used in reproduction programs and artificial insemination.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Identificador

JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY, SAO PAULO, v. 23, n. 3, supl. 1, Part 2, pp. 573-580, MAR, 2012

0103-5053

http://www.producao.usp.br/handle/BDPI/43218

10.1590/S0103-50532012000300026

http://dx.doi.org/10.1590/S0103-50532012000300026

Idioma(s)

eng

Publicador

SOC BRASILEIRA QUIMICA

SAO PAULO

Relação

JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY

Direitos

openAccess

Copyright SOC BRASILEIRA QUIMICA

Palavras-Chave #INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY #ATOMIC ABSORPTION SPECTROMETRY #BOVINE SEMEN #SAMPLE DILUTION #ESSENTIAL ELEMENTS #ATOMIC-ABSORPTION-SPECTROMETRY #TRACE-ELEMENTS #SEMINAL PLASMA #INFERTILE MEN #ICP-MS #ENVIRONMENTAL EXPOSURE #BRAZILIAN POPULATION #SHOOT PROCEDURE #SPERM QUALITY #WHOLE-BLOOD #CHEMISTRY, MULTIDISCIPLINARY
Tipo

article

original article

publishedVersion