Evaluation of Inductively Coupled Plasma Mass Spectrometry for Determining Ca, Cu, Fe, Mg, Mn, Se and Zn in Bovine Semen Samples using a Simple Sample Dilution Method
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
07/11/2013
07/11/2013
2012
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Resumo |
A simple and fast method for the determination of Ca, Cu, Fe, Mg, Mn, Se and Zn in bovine semen by quadrupole inductively coupled plasma spectrometry (q-ICP-MS) is described. Prior to analysis, samples (200 mu L) were diluted 1:50 in a solution containing 0.01% v/v Triton (R) X-100 and 0.5% v/v nitric acid and directly analyzed by ICP-MS. The limits of detection of the method are 0.3, 0.03, 0.2, 0.04, 0.04, 0.03 and 0.03 mu g L-1 for Ca-44, Cu-63, Fe-57, Mg-24, Zn-64, Se-82 and Mn-55, respectively. For purposes of comparison and method validation, four ordinary bovine semen samples were directly analyzed by ICP-MS and by flame atomic absorption spectrometry (FAAS) or graphite furnace atomic absorption spectrometry (GF AAS), with no statistical difference between the techniques at the 95% level when applying the t-test. Then, the proposed method was applied in the determinations of Ca, Cu, Fe, Mg, Mn, Se and Zn in collected samples of bovine semen from different breeds, which are used in reproduction programs and artificial insemination. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) |
Identificador |
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY, SAO PAULO, v. 23, n. 3, supl. 1, Part 2, pp. 573-580, MAR, 2012 0103-5053 http://www.producao.usp.br/handle/BDPI/43218 10.1590/S0103-50532012000300026 |
Idioma(s) |
eng |
Publicador |
SOC BRASILEIRA QUIMICA SAO PAULO |
Relação |
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY |
Direitos |
openAccess Copyright SOC BRASILEIRA QUIMICA |
Palavras-Chave | #INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY #ATOMIC ABSORPTION SPECTROMETRY #BOVINE SEMEN #SAMPLE DILUTION #ESSENTIAL ELEMENTS #ATOMIC-ABSORPTION-SPECTROMETRY #TRACE-ELEMENTS #SEMINAL PLASMA #INFERTILE MEN #ICP-MS #ENVIRONMENTAL EXPOSURE #BRAZILIAN POPULATION #SHOOT PROCEDURE #SPERM QUALITY #WHOLE-BLOOD #CHEMISTRY, MULTIDISCIPLINARY |
Tipo |
article original article publishedVersion |