Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT-PCR assay with in-house singleplex assays for comprehensive detection of human respiratory viruses


Autoria(s): Sakthivel, Senthilkumar K.; Whitaker, Brett; Lu, Xiaoyan; Oliveira, Danielle B. L.; Stockman, Lauren J.; Kamili, Shifaq; Oberste, M. Steven; Erdman, Dean D.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

06/11/2013

06/11/2013

2012

Resumo

Fast-track Diagnostics respiratory pathogens (FTDRP) multiplex real-time RT-PCR assay was compared with in-house singleplex real-time RT-PCR assays for detection of 16 common respiratory viruses. The FTDRP assay correctly identified 26 diverse respiratory virus strains, 35 of 41 (85%) external quality assessment samples spiked with cultured virus and 232 of 263 (88%) archived respiratory specimens that tested positive for respiratory viruses by in-house assays. Of 308 prospectively tested respiratory specimens selected from children hospitalized with acute respiratory illness, 270 (87.7%) and 265 (86%) were positive by FTDRP and in-house assays for one or more viruses, respectively, with combined test results showing good concordance (K=0.812, 95% CI = 0.786-0.838). Individual FTDRP assays for adenovirus, respiratory syncytial virus and rhinovirus showed the lowest comparative sensitivities with in-house assays, with most discrepancies occurring with specimens containing low virus loads and failed to detect some rhinovirus strains, even when abundant. The FTDRP enterovirus and human bocavirus assays appeared to be more sensitive than the in-house assays with some specimens. With the exceptions noted above, most FTDRP assays performed comparably with in-house assays for most viruses while offering enhanced throughput and easy integration by laboratories using conventional real-time PCR instrumentation. Published by Elsevier B.V.

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Identificador

JOURNAL OF VIROLOGICAL METHODS, AMSTERDAM, v. 185, n. 2, pp. 259-266, NOV, 2012

0166-0934

http://www.producao.usp.br/handle/BDPI/42252

10.1016/j.jviromet.2012.07.010

http://dx.doi.org/10.1016/j.jviromet.2012.07.010

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

AMSTERDAM

Relação

JOURNAL OF VIROLOGICAL METHODS

Direitos

closedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #RESPIRATORY VIRUS #REAL-TIME PCR #MULTIPLEX #SPECIMENS #PANEL #IDENTIFICATION #CORONAVIRUS #INFECTIONS #BIOCHEMICAL RESEARCH METHODS #BIOTECHNOLOGY & APPLIED MICROBIOLOGY #VIROLOGY
Tipo

article

original article

publishedVersion