Evaluation of the mutagenic activity of chrysin, a flavonoid inhibitor of the aromatization process
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
07/11/2013
07/11/2013
2012
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Resumo |
Chrysin is one of the natural flavonoids present in plants, and large amounts are present in honey and propolis. In addition to anticancer, antioxidation, and anti-inflammatory activities, chrysin has also been reported to be an inhibitor of aromatase, an enzyme converting testosterone into estrogen. The present study evaluated the mutagenicity of this flavonoid using micronucleus (MN) with HepG2 cells and Salmonella. Cell survival after exposure to different concentrations of chrysin was also determined using sulforhodamine B (SRB) colorimetric assay in HepG2 cells and the influence of this flavonoid on growth of cells in relation to the cell cycle and apoptosis. TheMN test showed that from 1 to 15 mu M of this flavonoid mutagenic activity was noted in HepG2 cells. The Salmonella assay demonstrated a positive response to the TA100 Salmonella strain in the presence or absence of S9, suggesting that this compound acted on DNA, inducing base pair substitution before or after metabolism via cytochrome P-450. The SRB assay illustrated that chrysin promoted growth inhibition of HepG2 cells in both periods studied (24 and 48 h). After 24 h of exposure it was noted that the most significant results were obtained with a concentration of 50 mu M, resulting in 83% inhibition and SubG0 percentage of 12%. After 48 h of incubation cell proliferation inhibition rates (97% at 50 mu M) were significantly higher. Our results showed that chrysin is a mutagenic and cytotoxic compound in cultured human HepG2 cells and Salmonella typhimurium. Although it is widely accepted that flavonoids are substances beneficial to health, one must evaluate the risk versus benefit relationship and concentrations of these substances to which an individual may be exposed. Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo Faculdade de Filosofia, Ciencias e Letras de Ribeirao Preto, Universidade de Sao Paulo Faculdade de Filosofia, Ciencias e Letras de Ribeirao Preto, Universidade de Sao Paulo FAPESP FAPESP CAPES CAPES |
Identificador |
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, PHILADELPHIA, v. 75, n. 16-17, Special Issue, supl. 1, Part 2, pp. 1000-1011, JAN-MAR, 2012 1528-7394 http://www.producao.usp.br/handle/BDPI/42789 10.1080/15287394.2012.696517 |
Idioma(s) |
eng |
Publicador |
TAYLOR & FRANCIS INC PHILADELPHIA |
Relação |
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES |
Direitos |
restrictedAccess Copyright TAYLOR & FRANCIS INC |
Palavras-Chave | #HUMAN LYMPHOCYTE-CULTURES #HUMAN HEPATOMA-CELLS #MICRONUCLEUS TEST #CYCLE ARREST #ASSAY #CARCINOGENS #DIETARY #DNA #CYTOTOXICITY #GENOTOXICITY #ENVIRONMENTAL SCIENCES #PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH #TOXICOLOGY |
Tipo |
article original article publishedVersion |