Development of a high sensitive automatic setup for screening of microcystins in surface waters by employing a LED-based photometric detector


Autoria(s): Vieira, Glaucia P.; Perdigao, Sheila R. W.; Fiore, Marli F.; Reis, Boaventura F.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

06/11/2013

06/11/2013

2012

Resumo

In this manuscript, an automatic setup for screening of microcystins in surface waters by employing photometric detection is described. Microcystins are toxins delivered by cyanobacteria within an aquatic environment, which have been considered strongly poisonous for humans. For that reason, the World Health Organization (WHO) has proposed a provisional guideline value for drinking water of 1 mu g L-1. In this work, we developed an automated equipment setup, which allows the screening of water for concentration of microcystins below 0.1 mu g V. The photometric method was based on the enzyme-linked immunosorbent assay (ELISA) and the analytical signal was monitored at 458 nm using a homemade LED-based photometer. The proposed system was employed for the detection of microcystins in rivers and lakes waters. Accuracy was assessed by processing samples using a reference method and applying the paired t-test between results. No significant difference at the 95% confidence level was observed. Other useful features including a linear response ranging from 0.05 up to 2.00 mu g L-1 (R-2 =0.999) and a detection limit of 0.03 mu g L-1 microcystins were achieved. (C) 2011 Elsevier B.V. All rights reserved.

CNPq [proc. 130127/2008-6, proc. 308299/2009-4, proc. 305043/2008-0]

CAPES

FAPESP

PRONEX/FAPESB

CNPq/INCTAA

Identificador

SENSORS AND ACTUATORS B-CHEMICAL, LAUSANNE, v. 161, n. 1, pp. 422-428, JAN 3, 2012

0925-4005

http://www.producao.usp.br/handle/BDPI/42210

10.1016/j.snb.2011.10.054

http://dx.doi.org/10.1016/j.snb.2011.10.054

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE SA

LAUSANNE

Relação

SENSORS AND ACTUATORS B-CHEMICAL

Direitos

closedAccess

Copyright ELSEVIER SCIENCE SA

Palavras-Chave #MICROCYSTINS #ENZYME-LINKED IMMUNOSORBENT ASSAY #SURFACE WATERS #LED-BASED PHOTOMETER #AUTOMATION #MULTICOMMUTED FLOW ANALYSIS #TANDEM MASS-SPECTROMETRY #SPECTROPHOTOMETRIC DETERMINATION #ANALYTICAL-CHEMISTRY #FLOW-ANALYSIS #TAP WATER #SYSTEM #EXTRACTION #STRATEGIES #MULTICOMMUTATION #HEPATOTOXINS #CHEMISTRY, ANALYTICAL #ELECTROCHEMISTRY #INSTRUMENTS & INSTRUMENTATION
Tipo

article

original article

publishedVersion