Previous illumination of a water soluble chlorine photosensitizer increases its cytotoxicity
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
06/11/2013
06/11/2013
2012
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Resumo |
Photodithazine (PDZ) is an N-methyl-D-glucosamine derivative of chlorine e6 that is water soluble and has an intense absorption in the range of 650-680 nm. PDZ photobleaching and photoproduct formation were induced by illumination with laser at two wavelengths: 514 nm (ion argon laser) as well as in 630 nm (dye laser). The time constants of PDZ photobleaching were: 18 min for 630 nm irradiation and 50 min for 514 nm irradiation, suggesting that degradation after irradiation with red light is faster than with green light. Photoproducts formation was evidenced by the appearance of a new absorption band at 668 nm with slight broaden of the Soret band, suggesting that there was no break of the macrocycle. The cytotoxicity of the photodegradated PDZ was investigated and showed to be lower in the dark and higher than non irradiated PDZ. These results may have important clinical implications for PDT such as the possibility to use the previously irradiated PDZ just before clinical application in order to get increased efficiency. CNPq CNPq FAPESP FAPESP J.C. Correa fellowship J.C. Correa fellowship |
Identificador |
LASER PHYSICS, NEW YORK, v. 22, n. 9, supl., Part 3, pp. 1387-1394, SEP, 2012 1054-660X http://www.producao.usp.br/handle/BDPI/42010 10.1134/S1054660X12090034 |
Idioma(s) |
eng |
Publicador |
MAIK NAUKA/INTERPERIODICA/SPRINGER NEW YORK |
Relação |
LASER PHYSICS |
Direitos |
restrictedAccess Copyright MAIK NAUKA/INTERPERIODICA/SPRINGER |
Palavras-Chave | #PHOTODYNAMIC THERAPY #PHOTOPRODUCT FORMATION #CELLS #SENSITIZERS #PHOTOSTABILITY #PHOTOTRANSFORMATIONS #PHOTODEGRADATION #PHOTOGEM(R) #PORPHYRINS #EFFICIENCY #OPTICS #PHYSICS, APPLIED |
Tipo |
article original article publishedVersion |