Previous illumination of a water soluble chlorine photosensitizer increases its cytotoxicity


Autoria(s): Correa, J. C.; Bagnato, Vanderlei Salvador; Imasato, H.; Perussi, J. R.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

06/11/2013

06/11/2013

2012

Resumo

Photodithazine (PDZ) is an N-methyl-D-glucosamine derivative of chlorine e6 that is water soluble and has an intense absorption in the range of 650-680 nm. PDZ photobleaching and photoproduct formation were induced by illumination with laser at two wavelengths: 514 nm (ion argon laser) as well as in 630 nm (dye laser). The time constants of PDZ photobleaching were: 18 min for 630 nm irradiation and 50 min for 514 nm irradiation, suggesting that degradation after irradiation with red light is faster than with green light. Photoproducts formation was evidenced by the appearance of a new absorption band at 668 nm with slight broaden of the Soret band, suggesting that there was no break of the macrocycle. The cytotoxicity of the photodegradated PDZ was investigated and showed to be lower in the dark and higher than non irradiated PDZ. These results may have important clinical implications for PDT such as the possibility to use the previously irradiated PDZ just before clinical application in order to get increased efficiency.

CNPq

CNPq

FAPESP

FAPESP

J.C. Correa fellowship

J.C. Correa fellowship

Identificador

LASER PHYSICS, NEW YORK, v. 22, n. 9, supl., Part 3, pp. 1387-1394, SEP, 2012

1054-660X

http://www.producao.usp.br/handle/BDPI/42010

10.1134/S1054660X12090034

http://dx.doi.org/10.1134/S1054660X12090034

Idioma(s)

eng

Publicador

MAIK NAUKA/INTERPERIODICA/SPRINGER

NEW YORK

Relação

LASER PHYSICS

Direitos

restrictedAccess

Copyright MAIK NAUKA/INTERPERIODICA/SPRINGER

Palavras-Chave #PHOTODYNAMIC THERAPY #PHOTOPRODUCT FORMATION #CELLS #SENSITIZERS #PHOTOSTABILITY #PHOTOTRANSFORMATIONS #PHOTODEGRADATION #PHOTOGEM(R) #PORPHYRINS #EFFICIENCY #OPTICS #PHYSICS, APPLIED
Tipo

article

original article

publishedVersion