Ether-A -go-go 1 (Eag1) Potassium Channel Expression in Dopaminergic Neurons of Basal Ganglia is Modulated by 6-Hydroxydopamine Lesion


Autoria(s): Ferreira, N. R.; Mitkovski, M.; Stuehmer, W.; Pardo, L. A.; Del Bel, E. A.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

06/11/2013

06/11/2013

2012

Resumo

The ether A go-go (Eag) gene encodes the voltage-gated potassium (K+) ion channel Kv10.1, whose function still remains unknown. As dopamine may directly affect K+ channels, we evaluated whether a nigrostriatal dopaminergic lesion induced by the neurotoxin 6-hydroxydopamine (6-OHDA) would alter Eag1-K+ channel expression in the rat basal ganglia and related brain regions. Male Wistar rats received a microinjection of either saline or 6-OHDA (unilaterally) into the medial forebrain bundle. The extent of the dopaminergic lesion induced by 6-OHDA was evaluated by apomorphine-induced rotational behavior and by tyrosine hydroxylase (TH) immunoreactivity. The 6-OHDA microinjection caused a partial or complete lesion of dopaminergic cells, as well as a reduction of Eag1+ cells in a manner proportional to the extent of the lesion. In addition, we observed a decrease in TH immunoreactivity in the ipsilateral striatum. In conclusion, the expression of the Eag1-K+-channel throughout the nigrostriatal pathway in the rat brain, its co-localization with dopaminergic cells and its reduction mirroring the extent of the lesion highlight a physiological circuitry where the functional role of this channel can be investigated. The Eag1-K+ channel expression in dopaminergic cells suggests that these channels are part of the diversified group of ion channels that generate and maintain the electrophysiological activity pattern of dopaminergic midbrain neurons.

FAPESP

FAPESP

CNPq

CNPq

CAPES/DAAD

CAPES/DAAD

Identificador

NEUROTOXICITY RESEARCH, NEW YORK, v. 21, n. 3, supl. 2, Part 1-2, pp. 317-333, APR, 2012

1029-8428

http://www.producao.usp.br/handle/BDPI/42320

10.1007/s12640-011-9286-3

http://dx.doi.org/10.1007/s12640-011-9286-3

Idioma(s)

eng

Publicador

SPRINGER

NEW YORK

Relação

NEUROTOXICITY RESEARCH

Direitos

closedAccess

Copyright SPRINGER

Palavras-Chave #DOPAMINE #6-OHDA #BASAL GANGLIA #POTASSIUM CHANNELS #EAG1 #NITRIC-OXIDE SYNTHASE #RAT STRIATAL NEURONS #PARKINSONS-DISEASE #SUBSTANTIA-NIGRA #K+-CHANNELS #FUNCTIONAL EXPRESSION #NIGROSTRIATAL PATHWAY #MIDBRAIN NEURONS #6-OHDA LESIONS #ADULT-RAT #NEUROSCIENCES
Tipo

article

original article

publishedVersion