A Combined Study Using Ligand-Based Design, Synthesis, and Pharmacological Evaluation of Analogues of the Acetaminophen Ortho-Regioisomer with Potent Analgesic Activity


Autoria(s): Queiroz, Luana M. D.; Rocha, Josmar Rodrigues da; Leitão, Andrei; Montanari, Carlos Alberto; Silva, Albérico Borges Ferreira da; Sousa, Pergentino J. C.; Borges, Rosivaldo dos Santos
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

07/11/2013

07/11/2013

2012

Resumo

A ligand-based drug design study was performed to acetaminophen regioisomers as analgesic candidates employing quantum chemical calculations at the DFT/B3LYP level of theory and the 6-31G* basis set. To do so, many molecular descriptors were used such as highest occupied molecular orbital, ionization potential, HO bond dissociation energies, and spin densities, which might be related to quench reactivity of the tyrosyl radical to give N-acetyl-p-benzosemiquinone-imine through an initial electron withdrawing or hydrogen atom abstraction. Based on this in silico work, the most promising molecule, orthobenzamol, was synthesized and tested. The results expected from the theoretical prediction were confirmed in vivo using mouse models of nociception such as writhing, paw licking, and hot plate tests. All biological results suggested an antinociceptive activity mediated by opioid receptors. Furthermore, at 90 and 120 min, this new compound had an effect that was comparable to morphine, the standard drug for this test. Finally, the pharmacophore model is discussed according to the electronic properties derived from quantum chemistry calculations.

CAPES

CNPq

FAPESPA

PARDUFPA

PARD-UFPA

Identificador

CHEMICAL BIOLOGY & DRUG DESIGN, HOBOKEN, v. 80, n. 1, supl. 2, Part 1-2, pp. 100-106, JUL, 2012

1747-0277

http://www.producao.usp.br/handle/BDPI/42738

10.1111/j.1747-0285.2012.01372.x

http://dx.doi.org/10.1111/j.1747-0285.2012.01372.x

Idioma(s)

eng

Publicador

WILEY-BLACKWELL

HOBOKEN

Relação

Chemical Biology and Drug Design

Direitos

closedAccess

Copyright WILEY-BLACKWELL

Palavras-Chave #ACETAMINOPHEN #ANALGESIC EFFECT #LIGAND-BASED DRUG DESIGN #MOLECULAR MODELING #OPIOID DERIVATIVES #PERFORMANCE LIQUID-CHROMATOGRAPHY #FORMALIN TEST #ACETIC-ACID #3,5-DISUBSTITUTED ANALOGS #3,5-DIALKYL DERIVATIVES #PARACETAMOL #MICE #OXIDATION #HEPATOTOXICITY #INHIBITION #BIOCHEMISTRY & MOLECULAR BIOLOGY #CHEMISTRY, MEDICINAL
Tipo

article

original article

publishedVersion