11-Oxoaerothionin isolated from the marine sponge Aplysina fistularis shows anti-inflammatory activity in LPS-stimulated macrophages
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
06/11/2013
06/11/2013
2012
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Resumo |
Marine sponges of the order Verongida are a rich source of biologically active bromotyrosine-derived secondary metabolites. However, none of these compounds are known to display anti-inflammatory activity. In the present investigation, we report the anti-inflammatory effects of 11-oxoaerothionin isolated from the Verongida sponge Aplysina fistularis. When RAW264.7 cells and primary macrophages were preincubated with 11-oxoaerothionin and stimulated with LPS (lipopolysaccharide), a concentration-dependent inhibition of iNOS (inducible nitric oxide synthase) protein and NO2- (Nitrite) production were observed. The same effect was observed when proinflammatory cytokines and PGE(2) (Prostaglandin E2) production was evaluated. In summary, we demonstrated that in the presence of LPS, 11-oxoaerothionin suppresses NO2 and iNOS expression as well as inflammatory cytokines and PGE(2). Fundaao de Amparo a Pesquisa do Estado de Sao Paulo BIOTA/BIOprospecTA program [2005/60175-2, 2010/50190-2] Fundaao de Amparo a Pesquisa do Estado de Sao Paulo BIOTA/BIOprospecTA program Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (Brazil) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil [CNPq-471509/2006-0] |
Identificador |
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, LONDON, v. 34, n. 6, supl. 4, Part 1-2, pp. 919-924, DEC, 2012 0892-3973 http://www.producao.usp.br/handle/BDPI/42683 10.3109/08923973.2012.679984 |
Idioma(s) |
eng |
Publicador |
INFORMA HEALTHCARE LONDON |
Relação |
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY |
Direitos |
closedAccess Copyright INFORMA HEALTHCARE |
Palavras-Chave | #PROSTAGLANDIN E2 #MACROPHAGES #INFLAMMATORY CYTOKINES #NITRIC OXIDE #NF-KAPPA B AND LIPOPOLYSACCHARIDE #TOLL-LIKE RECEPTORS #NATURAL-PRODUCTS #NITRIC-OXIDE #LEUKOTRIENE SYNTHESIS #ALVEOLAR MACROPHAGES #CYCLIC-AMP #LIPOPOLYSACCHARIDE #PHAGOCYTOSIS #INDUCTION #IMMUNOLOGY #PHARMACOLOGY & PHARMACY #TOXICOLOGY |
Tipo |
article original article publishedVersion |