Prognostication of Soft Tissue Sarcomas Based on Chromosome 17q Gene and Protein Status: Evaluation of TOP2A, HER-2/neu, and Survivin
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
06/11/2013
06/11/2013
2012
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Resumo |
Topoisomerase 2 alpha (), HER-2/ and are genes that lie on chromosome 17 and correlate with the prognosis and prediction of target-driven therapy against tumors. In a previous study, we showed that TOP2A transcripts levels were significantly higher in soft tissue sarcomas (STS) than in benign tumors and desmoid-type fibromatoses (FM). Because these genes have been insufficiently examined in STS, we aimed to identify alterations in TOP2A and HER-2 expression by fluorescent in situ hybridization and immunohistochemistry, as well as that of survivin, and correlate them with clinicopathologic findings to assess their prognostic value. Eighteen FM and 244 STS were included. Fluorescent in situ hybridization and immunohistochemistry were performed on a tissue microarray. TOP2A and survivin were more highly expressed in sarcomas than in FM. TOP2A was an independent predictor of an unfavorable prognosis; it was combined with formerly established prognostic factors (primarily histologic grade and tumor size at diagnosis) to create a prognostic index that evaluated overall survival. Gene amplification/polysomy (13%) did not correlate with protein overexpression. Survivin and HER-2 expression were not associated with patient outcomes. These findings might become valuable in the management of patients with STS and possibly in the prospective evaluation of responses to new target-driven therapies. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Brazil FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil) |
Identificador |
ANNALS OF SURGICAL ONCOLOGY, NEW YORK, v. 19, n. 6, supl. 1, Part 1, pp. 1790-1799, JUN, 2012 1068-9265 http://www.producao.usp.br/handle/BDPI/42511 10.1245/s10434-011-2184-3 |
Idioma(s) |
eng |
Publicador |
SPRINGER NEW YORK |
Relação |
ANNALS OF SURGICAL ONCOLOGY |
Direitos |
closedAccess Copyright SPRINGER |
Palavras-Chave | #TOPOISOMERASE-II-ALPHA #IN-SITU HYBRIDIZATION #NERVE SHEATH TUMORS #BREAST-CANCER #SYNOVIAL SARCOMA #ELEVATED EXPRESSION #COLORECTAL-CANCER #CELL-LINES #AMPLIFICATION #HER2 #ONCOLOGY #SURGERY |
Tipo |
article original article publishedVersion |