Molecular Characterization and Physical Mapping of Two Classes of 5S rDNA in the Genomes of Gymnotus sylvius and G. inaequilabiatus (Gymnotiformes, Gymnotidae)


Autoria(s): Scacchetti, P. C.; Alves, J. C. P.; Utsunomia, R.; Claro, F. L.; Toledo, Lurdes Foresti de Almeida; Oliveira, C.; Foresti, F.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

06/11/2013

06/11/2013

2012

Resumo

The nucleotide sequences of the 5S rRNA multigene family and their distribution across the karyotypes in 2 species of Gymnotiformes, genus Gymnotus (G. sylvius and G. inaequilabiatus) were investigated by means of fluorescence in situ hybridization (FISH). The results showed the existence of 2 distinct classes of 5S rDNA sequences in both species: class I and class II. A high conservative pattern of the codifying region of the 5S rRNA gene was identified, contrasting with significant alterations detected in the nontranscribed spacer (NTS). The presence of TATA-like sequences along the NTS of both species was an expected occurrence, since such sequences have been associated with the regulation of the gene expression. FISH using 5S rDNA class I and class II probes revealed that both gene classes were collocated in the same chromosome pair in the genome of G. sylvius, while in that of G. inaequilabiatus, class II appeared more disperse than class I. Copyright (C) 2012 S. Karger AG, Basel

CNPq

CNPq

CAPES

CAPES

FAPESP

FAPESP

Identificador

CYTOGENETIC AND GENOME RESEARCH, BASEL, v. 136, n. 2, supl. 1, Part 1, pp. 131-137, MAR, 2012

1424-8581

http://www.producao.usp.br/handle/BDPI/42225

10.1159/000335658

http://dx.doi.org/10.1159/000335658

Idioma(s)

eng

Publicador

KARGER

BASEL

Relação

CYTOGENETIC AND GENOME RESEARCH

Direitos

restrictedAccess

Copyright KARGER

Palavras-Chave #COMPARATIVE GENE MAPPING #DISPERSED REPETITIVE SEQUENCE FAMILY #FISH CYTOGENETICS #KNIFE-FISHES #MINOR RIBOSOMAL GENES #ATLANTIC SALMON #RIBOSOMAL DNA #ORGANIZATION #GENES #FISH #SEQUENCES #5S-RDNA #HYBRID #CARAPO #PISCES #CELL BIOLOGY #GENETICS & HEREDITY
Tipo

article

original article

publishedVersion