Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
06/11/2013
06/11/2013
2012
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Resumo |
Antitumor activities have been described in selol, a hydrophobic mixture of molecules containing selenium in their structure, and also in maghemite magnetic nanoparticles (MNPs). Both selol and MNPs were co-encapsulated within poly(lactic-co-glycolic acid) (PLGA) nanocapsules for therapeutic purposes. The PLGA-nanocapsules loaded with MNPs and selol were labeled MSE-NC and characterized by transmission and scanning electron microscopy, electrophoretic mobility, photon correlation spectroscopy, presenting a monodisperse profile, and positive charge. The antitumor effect of MSE-NC was evaluated using normal (MCF-10A) and neoplastic (4T1 and MCF-7) breast cell lines. Nanocapsules containing only MNPs or selol were used as control. MTT assay showed that the cytotoxicity induced by MSE-NC was dose and time dependent. Normal cells were less affected than tumor cells. Cell death occurred mainly by apoptosis. Further exposure of MSE-NC treated neoplastic breast cells to an alternating magnetic field increased the antitumor effect of MSE-NC. It was concluded that selol-loaded magnetic PLGA-nanocapsules (MSE-NC) represent an effective magnetic material platform to promote magnetohyperthermia and thus a potential system for antitumor therapy. Rede CON-NANO/CAPES Rede CONNANO/CAPES INCTNanobiotechnologia/MCT/CNPq INCT-Nanobiotechnologia/MCT/CNPq FAP-DF FAPDF FAPESP FAPESP [2009/13208-3] DPP/UnB DPP/UnB CNANO/IB/UnB CNANO/IB/UnB Polish State Committee for Scientific Research Polish State Committee for Scientific Research [N N 202 166440, N N 405 360639] |
Identificador |
INTERNATIONAL JOURNAL OF NANOMEDICINE, ALBANY, v. 7, n. 2, supl., Part 3, pp. 5287-5299, FEB, 2012 1178-2013 http://www.producao.usp.br/handle/BDPI/42224 10.2147/IJN.S35279 |
Idioma(s) |
eng |
Publicador |
DOVE MEDICAL PRESS LTD ALBANY |
Relação |
INTERNATIONAL JOURNAL OF NANOMEDICINE |
Direitos |
closedAccess Copyright DOVE MEDICAL PRESS LTD |
Palavras-Chave | #PLGA-NANOCAPSULE #CANCER #MAGHEMITE NANOPARTICLE #MTT #DRUG DELIVERY #SELENIUM #DRUG-DELIVERY SYSTEM #SELENIUM NANOPARTICLES #ANTICANCER EFFICACY #FLUID HYPERTHERMIA #CANCER-TREATMENT #CELLS #APOPTOSIS #POLYSACCHARIDE #ADRIAMYCIN #INDUCTION #NANOSCIENCE & NANOTECHNOLOGY #PHARMACOLOGY & PHARMACY |
Tipo |
article original article publishedVersion |