Antioxidant defenses and biochemical changes in the neotropical fish pacu, Piaractus mesopotamicus: Responses to single and combined copper and hypercarbia exposure
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
14/10/2013
14/10/2013
2012
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Resumo |
This study investigated the potentially detrimental effects of copper and elevated aquatic CO2 (hypercarbia), alone or in combination, on pacu, Piaractus mesopotamicus. Fish were exposed for 48 h to control (no copper addition in normocarbia), to 400 mu g Cu2+L-1, to hypercarbic (1% CO2; PCO2=6.9 mm Hg) water and to 400 mu g Cu2+L-1+ hypercarbia. In liver the single factors caused an increase in lipid hydroperoxide concentration that was not observed when the factors were combined. Copper exposure elicited increased hepatic superoxide dismutase activity, irrespective of aquatic CO2 level. On the other hand, the effects of copper on hepatic glutathione peroxidase activity were dependent on water CO2 levels. The two stressors combined did not affect hepatic catalase activity. Hypercarbic water caused a decline in plasma glucose concentration, but this was not observed when hypercarbia was combined with copper exposure. Copper caused a decrease in branchial Na+/K+-ATPase activity that was independent of water CO2 level. Copper caused an increase in branchial metallothionein concentration that was independent of water CO2 level. Thus, branchial metallothionein and Na+/K+-ATPase were effective biomarkers of copper exposure that were not affected by water CO2 level. (C) 2012 Elsevier Inc. All rights reserved. CNPq CNPq Aquapeixe Aquaculture (Conchal, SP, Brazil) Aquapeixe Aquaculture (Conchal, SP, Brazil) Sao Paulo State Research Foundation (FAPESP) Sao Paulo State Research Foundation - FAPESP [Proc. 03/06105-7] |
Identificador |
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, NEW YORK, v. 156, n. 41367, supl. 1, Part 6, pp. 178-186, NOV, 2012 1532-0456 http://www.producao.usp.br/handle/BDPI/34319 10.1016/j.cbpc.2012.07.002 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCIENCE INC NEW YORK |
Relação |
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY |
Direitos |
closedAccess Copyright ELSEVIER SCIENCE INC |
Palavras-Chave | #ANTIOXIDANT DEFENSES #HEMATOLOGY #HYPERCARBIA #METALLOTHIONEIN #NA+/K+-ATPASE #SALMO-SALAR L. #TILAPIA OREOCHROMIS-MOSSAMBICUS #EEL ANGUILLA-ANGUILLA #FRESH-WATER ANIMALS #NUCELLA-LAPILLUS L. #CARBON-DIOXIDE #METALLOTHIONEIN INDUCTION #PROCHILODUS-SCROFA #RAINBOW-TROUT #ACIPENSER-TRANSMONTANUS #BIOCHEMISTRY & MOLECULAR BIOLOGY #ENDOCRINOLOGY & METABOLISM #TOXICOLOGY #ZOOLOGY |
Tipo |
article original article publishedVersion |