The role of cytoskeleton, components of inositol phospholipid signaling pathway and iron in Ehrlichia canis in vitro proliferation


Autoria(s): Levenhagen, Marcelo Arantes; Alves, Rosiane Nascimento; Rieck, Susana Elisa; Labruna, Marcelo Bahia; Beletti, Marcelo Emilio
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

05/11/2013

05/11/2013

2012

Resumo

Ehrlichia canis, etiologic agent of Canine Monocytic Ehrlichiosis, is an obligatory intracellular bacterium that parasitizes monocytes and macrophages. In this study we analyzed the role of the cytoskeleton specifically actin microfilaments and microtubules, components of inositol phospholipid signaling pathway such as phospholipase C (PLC), protein kinase (PTK) and calcium channels as well as the role of iron in the E. canis proliferation in DH82 cells. Different inhibitory compounds were used for each component: Cytochalasin D (inhibits actin polymerization), Nocodazole (inhibits microtubule polymerization), Neomycin (PLC inhibitor), Genistein (PTK inhibitor), Verapamil (calcium channel blocker) and Deferoxamine (iron chelator). We observed a significant decrease in the total number of bacteria in infected cells treated suggesting that these cellular components analized are essentials to E. canis proliferation.

Identificador

VETERINARY RESEARCH COMMUNICATIONS, DORDRECHT, v. 36, n. 3, supl. 1, Part 3, pp. 195-199, SEP, 2012

0165-7380

http://www.producao.usp.br/handle/BDPI/41844

10.1007/s11259-012-9525-y

http://dx.doi.org/10.1007/s11259-012-9525-y

Idioma(s)

eng

Publicador

SPRINGER

DORDRECHT

Relação

VETERINARY RESEARCH COMMUNICATIONS

Direitos

closedAccess

Copyright SPRINGER

Palavras-Chave #CELL SIGNALING #EHRLICHIA CANIS #DH82 CELLS #CHAFFEENSIS #ANAPLASMATACEAE #TRANSFERRIN #VETERINARY SCIENCES
Tipo

article

original article

publishedVersion