Gene expression during early somatic embryogenesis in Brazilian pine (Araucaria angustifolia (Bert) O. Ktze)


Autoria(s): Schloegl, Paulo Sergio; Wendt dos Santos, Andre Luis; Vieira, Leila do Nascimento; Floh, Eny Iochevet Segal; Guerra, Miguel Pedro
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

06/11/2013

06/11/2013

2012

Resumo

Brazilian pine (Araucaria angustifolia (Bert) O. Ktze) is the only native conifer species with economic importance in Brazil. Recently, due to intensive exploitation Brazilian pine was included in the official list of endangered Brazilian plants, under the "vulnerable" category. Biotechnology tools like somatic embryogenesis (SE) are potentially useful for mass clonal propagation and ex situ conservation strategies of commercial and endangered plant species. In spite of that, numerous obstacles still hamper the full application of SE technology for a wider range of species, including Brazilian pine. To enhance somatic embryogenesis in Brazilian pine and to gain a better understanding of the molecular events associated with somatic embryo development, we analyzed the steady-state transcript levels of genes known to regulate somatic embryogenesis using semiquantitative reverse transcription polymerase chain reaction (sqRT-PCR). These genes included Argonaute (AaAGO), Cup-shaped cotyledon1 (AaCUC), wushel-related WOX (AaWOX), a S-locus lectin protein kinase (AaLecK), Scarecrow- like (AaSCR), Vicilin 7S (AaVIC), Leafy Cotyledon 1 (AaLEC), and a Reversible glycosylated polypeptide (AaRGP). Expression patterns of these selected genes were investigated in embryogenic cultures undergoing different stages of embryogenesis, and all the way to maturation. Up-regulation of AaAGO, AaCUC, AaWOX, AaLecK, and AaVIC was observed during transition of somatic embryos from stage I to stage II. During the maintenance phase of somatic embryogenesis, expression of AaAGO and AaSCR, but not AaRPG and AaLEC genes was influenced by presence/ absence of plant growth regulators, both auxins and cytokinins. The results presented here provide new insights on the molecular mechanisms responsible for somatic embryo formation, and how selected genes may be used as molecular markers for Brazilian pine embryogenesis.

National Counsel of Technological and Scientific Development (CNPq)

National Counsel of Technological and Scientific Development (CNPq)

CAPES

CAPES

Santa Catarina State Research Agency (FAPESC)

Santa Catarina State Research Agency (FAPESC) [152306/2007-2]

ALWS

ALWS [02437/09-0]

Identificador

PLANT CELL TISSUE AND ORGAN CULTURE, DORDRECHT, v. 108, n. 1, supl. 4, Part 1, pp. 173-180, JAN, 2012

0167-6857

http://www.producao.usp.br/handle/BDPI/42050

10.1007/s11240-011-0023-7

http://dx.doi.org/10.1007/s11240-011-0023-7

Idioma(s)

eng

Publicador

SPRINGER

DORDRECHT

Relação

PLANT CELL TISSUE AND ORGAN CULTURE

Direitos

closedAccess

Copyright SPRINGER

Palavras-Chave #GENE EXPRESSION #ARAUCARIACEAE #SOMATIC EMBRYOGENESIS #SQRT-PCR #EMBRYO DEVELOPMENT #SHOOT MERISTEM #PICEA-ABIES #MOLECULAR-BIOLOGY #SEED DEVELOPMENT #ABSCISIC-ACID #SPRUCE #ARABIDOPSIS #MATURATION #PROTEIN #BIOTECHNOLOGY & APPLIED MICROBIOLOGY #PLANT SCIENCES
Tipo

article

original article

publishedVersion