Endo-xylanase GH11 activation by the fungal metabolite eugenitin
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
|---|---|
| Data(s) |
06/11/2013
06/11/2013
2012
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| Resumo |
Eugenitin, a chromone derivative and a metabolite of the endophyte Mycoleptodiscus indicus, at 5 mM activated a recombinant GH11 endo-xylanase by 40 %. The in silico prediction of ligand-binding sites on the three-dimensional structure of the endo-xylanase revealed that eugenitin interacts mainly by a hydrogen bond with a serine residue and a stacking interaction of the heterocyclic aromatic ring system with a tryptophan residue. Eugenitin improved the GH11 endo-xylanase activity on different substrates, modified the optimal pH and temperature activities and slightly affected the kinetic parameters of the enzyme. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) |
| Identificador |
BIOTECHNOLOGY LETTERS, DORDRECHT, v. 34, n. 8, supl. 1, Part 1, pp. 1487-1492, AUG, 2012 0141-5492 http://www.producao.usp.br/handle/BDPI/42061 10.1007/s10529-012-0918-3 |
| Idioma(s) |
eng |
| Publicador |
SPRINGER DORDRECHT |
| Relação |
BIOTECHNOLOGY LETTERS |
| Direitos |
closedAccess Copyright SPRINGER |
| Palavras-Chave | #ASPERGILLUS NIDULANS #ASPERGILLUS NIVEUS #EUGENITIN #GH11 ACTIVATION #GH11 ENDO-XYLANASE #MYCOLEPTODISCUS INDICUS #PROTEIN #FAMILIES #SITES #BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Tipo |
article original article publishedVersion |
