Infrared (810-nm) low-level laser therapy on rat experimental knee inflammation
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
07/11/2013
07/11/2013
2012
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Resumo |
Arthritis of the knee is the most common type of joint inflammatory disorder and it is associated with pain and inflammation of the joint capsule. Few studies address the effects of the 810-nm laser in such conditions. Here we investigated the effects of low-level laser therapy (LLLT; infrared, 810-nm) in experimentally induced rat knee inflammation. Thirty male Wistar rats (230-250 g) were anesthetized and injected with carrageenan by an intra-articular route. After 6 and 12 h, all animals were killed by CO(2) inhalation and the articular cavity was washed for cellular and biochemical analysis. Articular tissue was carefully removed for real-time PCR analysis in order to evaluate COX-1 and COX-2 expression. LLLT was able to significantly inhibit the total number of leukocytes, as well as the myeloperoxidase activity with 1, 3, and 6 J (Joules) of energy. This result was corroborated by cell counting showing the reduction of polymorphonuclear cells at the inflammatory site. Vascular extravasation was significantly inhibited at the higher dose of energy of 10 J. Both COX-1 and 2 gene expression were significantly enhanced by laser irradiation while PGE(2) production was inhibited. Low-level laser therapy operating at 810 nm markedly reduced inflammatory signs of inflammation but increased COX-1 and 2 gene expression. Further studies are necessary to investigate the possible production of antiinflammatory mediators by COX enzymes induced by laser irradiation in knee inflammation. FAPESP [2005/02117-6] FAPESP CAPES CAPES |
Identificador |
LASERS IN MEDICAL SCIENCE, LONDON, v. 27, n. 1, supl. 1, Part 1, pp. 71-78, JAN, 2012 0268-8921 http://www.producao.usp.br/handle/BDPI/43030 10.1007/s10103-011-0906-1 |
Idioma(s) |
eng |
Publicador |
SPRINGER LONDON LTD LONDON |
Relação |
LASERS IN MEDICAL SCIENCE |
Direitos |
closedAccess Copyright SPRINGER LONDON LTD |
Palavras-Chave | #LLLT #LASER #ARTHRITIS #INFLAMMATION #KNEE #PLACEBO-CONTROLLED TRIALS #RHEUMATOID-ARTHRITIS #DISEASE-ACTIVITY #INTERLEUKIN-6 #PAIN #OSTEOARTHRITIS #METABOLISM #EXPRESSION #RESOLUTION #CARTILAGE #ENGINEERING, BIOMEDICAL #SURGERY |
Tipo |
article original article publishedVersion |